ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 16 of 68
УЖМБС 2021, 6(5): 110–115
Experimental Medicine and Morphology

Characteristics of Muscle Recovery under Experimental Post-Traumatic Contracture

Pankevych V. V. 1, Matolych U. D. 1, Ushtan S. V. 1, Putko Z. P. 2, Dmytryshyn Yu. B. 2

The frequency of traumatic injuries of the maxillofacial area and the degree of their severity, have been increasing in recent years. The introduction of modern methods of treatment made it possible to improve the results of treatment and shorten its duration. Myogenic contractures develop as a complication after facial injuries, soft tissue injuries, in particular, masticatory muscles. The gastrocnemius muscle of a rat has the same striated structure as the pterygoideus medialis muscle of a person. The purpose of the work is to study the regeneration of striatal muscles with post-traumatic contractures on the basis of combined treatment of laboratory rats. Materials and methods. The study was carried out on 45 sexually mature 12 months old outbred white male rats, which weighed 180-200 g. In animals, reflex post-traumatic muscle contractures (traumatic myositis) resulting from the slaughter of calf muscles (without violating the integrity of muscle tissue) were simulated on the posterior left leg. The rats were divided into two groups. Group 1 included 21 animals that did not receive treatment and whose wounds healed by secondary intention. Group 2 included 24 rats that received combined treatment: on the 2nd day after surgery – general (oral) administration of the drug with the active substance cyclobenzaprine hydrochloride – 0.15 mg, once a day with daily procedures of magnetic laser therapy on the affected hind limb. Results and discussion. Depending on the signs of pain behavior (changes in behavior, restriction of movement, increased sensitivity, pain), the intensity of myogenic pain syndrome was assessed (low, moderate, high, very high). It was found that the use of combined treatment (magnetic laser therapy and the use of cyclobenzaprine hydrochloride) led to the normalization of behavioral and motor reactions by the 28th day: 56% of rats didn’t feel any pain and 44% had a low level of pain. The animals with the same trauma, which weren’t treated, showed a high and moderate level of pain. On the 28th day these animals could lean on their hind limbs while they were moving. However, they could not fully move due to a sharp restriction of their left hind limb function. The obtained results are the basis for the development of new approaches to improving the methods of prevention and rehabilitation treatment of contractures using magnetic laser therapy and central muscle relaxants. Conclusion. It was found that the use of magnetic laser therapy and cyclobenzaprine hydrochloride inhibited the development of an acute inflammatory reaction in the damaged muscle tissue of the hind limb of animals, accelerated and optimized reparative processes, which prevented excessive, functionally significant growth of connective tissue

Keywords: muscles, post-injection contractures, post-traumatic contractures, muscle relaxants, magnetic laser therapy

Full text: PDF (Ukr) 280K

  1. Matolych UD, Ushtan SV, Nazarevych MR, Kaminskyy MV, Kaminskyy VI. Retrospektyvnyy analiz struktury travmatychnykh poshkodzhen shchelepno-lytsevoyi dilyanky u m. Lvovi v period za 2016-2018 roky [Retrospective Analysis of the Structures of Traumatic Damages in the Maxillofacial Area in Lviv in the Period from 2016 to 2018]. UJMBS. 2019; 4(6): 239-244. [Ukrainian].
  2. Dydykin SS, Somkin VA, Ibrahimov ZI. Topohrafiko-atomichna budova krylo-nyzhnoshchelepnoho prostoru i yoho rol v rozvytku postin'yektsiynykh kontraktury nyzhnoi shchelepy [Topographic and atomic structure of the pterygomandibular space and its role in the development of postinjection contracture of the mandible]. Reports Vinnytsia National Med University. 2006; 10(2): 325-6. [Ukrainian]
  3. Zarins Uldis. Anatomy of Facial Expression. Anatomy Next Inc; 2014. 222 p.
  4. Krivoshchapov AV. Osobennosti stroeniya kozhi i skeletnykh myshts krys v norme i pri vospalenii [Feature of structure of skin and skeletal muscles of rats in a norm and at inflammation]. Bulletin of problems biology and medicine. 2011; 2 (2): 152-4. [Russian]
  5. Matsehora NA, Shkurenko OO, Lekan OYa, Baburina OA. Efektyvnist zastosuvannya haloaerozolnoyi ta mahnitolazernoyi terapiyi u vidnovlyuvalnomu likuvanni khvorykh na bronkhialnu astmu u spolucheni z ishemichnoyu khvoroboyu sertsya [Efficiency of the application of haloaerosol and magnitolaser therapy in the rehabilitative treatment of patients with bronchial asthma in combination]. Actual problems of transport medicine. 2019; 4(58): 64-72. [Ukrainian]
  6. Fedorov SM. Suchasni pohlyady na mahnitolazernu i fotomahnitnuterapiyu ta yikh zastosuvannya v nevrolohichniy praktytsi [The modern concept of magnetic-laser and photomagnetic therapy and its use in neurological practice]. Photobiology and Photomedicine. 2016; 1(2): 38-50. [Ukrainian]
  7. Tereshchenko O. Miorelaksanty u likuvanni nespetsyfichnoho bolyu u nyzhniy chastyni spyny [Muscle relaxants in the treatment of nonspecific lower back pain]. NeuroNews: psychoneurology and neuropsychiatry. 2017; 3(87): 46-8. [Ukrainian]
  8. Oros MM. Mioryks: yedynyy v Ukraini miorelaksant prolonhovanoi diyi dlya usunennya bolisnoho m’yazovoho spazmu [Myorix: the only long-acting muscle relaxant in Ukraine to eliminate painful muscle spasm]. Nevrolohiya, Psykhiatriya, Psykhoterapiya. 2018; 1(44): 18. [Ukrainian]
  9. Fedyachkin AN, Makarov IV, Yamshchikov NV, Tulaeva ON. Vliyanie povyshennoy sily tyazhesti na sostoyanie tkani skeletnykh myshts i mikrotsirkulyatornogo rusla v usloviyakh eksperimentalnogo ateroskleroza [Influence of increased gravity on the condition of skeletal muscle tissue and microcirculatory tract in the conditions of experimental atherosclerosis]. Health Edu Millennium. 2007; 3(9): 312-3. [Russian]
  10. Flecknell PA, Liles JH. Evaluation of locomotor activity and food and water consumption as a method of assessing postoperative pain in rodents. Anim Pain. 1992; 1: 482-8.
  11. Rtayl RA. histomorfometrychnyy analiz riznykh faz reheneratsiyi skeletnoi muskulatury shchuriv pislya nanesennya mekhanichnoi travmy [Histomorphometric Analysis of Different Phases of Skeletal Muscle Regeneration after Mechanical Injury]. UJMBS. 2020; 5(1): 239-244. [Ukrainian].
  12. Rozova KV, Bolhova TV, Tymoshenko KR, Vinnychuk YuD. Perebudova tkanyn skeletnykh m’yaziv, lehen ta sertsya shchuriv za umov hipoksiyi navantazhennya v eksperymenti [Restructuring of skeletal muscle, lung and heart tissues of rats under hypoxia training]. Physiological J. 2016; 62(6): 72-80. [Ukrainian].
  13. Veresiuk TO, Selskyy PR, Televiak AT, Selskyy BP. Morfolohichni zminy u skeletnykh m’yazakh zadnikh kintsivok shchuriv pry hostriy ishemiyi –reperfuziyi, sprychyneniy nakladannyam arterialnoho turniketa, ta yiyi korektsiyi karbatsetamom [Morphological changes in skeletal muscles of the hind limbs of rats in acute ischemia-reperfusion caused by arterial tourniquet application, and its correction by carbacetam]. Bulletin of medical and biological research. 2020; 3: 16-23. [Ukrainian].
  14. Pouremadi N, Motaghi A, Safdari R, Zarean P, Rashad A, Zarean P, et al. Clinical Outcomes of Low– level Laser Therapy in Management of Advanced Implant Surgery Complications: A Comparative Clinical Study. J Contemp Dent Pract. 2019; 20(1): 78–82.
  15. Dima R, Tieppo Francio V, Towery C, Davani S. Review of Literature on Low-level Laser Therapy Benefits for Nonpharmacological Pain Control in Chronic Pain and Osteoarthritis. Altern Ther Health Med. 2018; 24(5): 8-10.
  16. Zaychenko HV, Plyushch SI, Sinitsyna OS. Mistse tsentralnykh miorelaksantiv u likuvanni nespetsyfichnoho bolyu v spyni [Place of centrally acting muscle relaxants in the treatment of non-specific back pain]. INJ. 2018; 4(98): 63-67. [Ukrainian].
  17. Polyakova DS. Tsyklobenzapryn prolonhovanoi diyi u likuvanni bolyu u spyni ta shyi [Cyclobenzaprine prolonged action in the treatment of back and neck pain].Ukr Med J. 2020; 1(3): 69-71. [Ukrainian]