The purpose of the study was to detect ability to form biofilms by microorganisms that cause respiratory tract infections. Materials and methods. The study involved 97 strains of microorganisms. Microorganisms were isolated from children with respiratory tract infections. All strains, isolated from patients, were able to form biofilms. There were 44 strains of S. aureus (from patients with pneumonia – 13 strains, from patients with other respiratory diseases – 31), 34 strains of S. pneumoniae (pneumonia – 27 strains, other respiratory diseases – 7), 13 strains of K. pneumoniae (pneumonia – 6 strains, other respiratory diseases – 7), 6 strains of P. aeruginosa (pneumonia – 5 strains, other respiratory diseases – 1). Children were treated at the pulmonary department and intensive care unit in Kharkiv Regional Children's Clinical Hospital. Results and discussion. The optical density of primary biofilms formed by Gram-positive microorganisms was 1.33±0.24 Units of OD, and their secondary biofilms was 0.32±0.10 Units of OD. In patients with pneumonia optical density of primary biofilms of Gram-positive microorganisms was 1.48±0.21 Units of OD and of secondary biofilms was 0.30±0.08 Units of OD. Optical density of primary biofilms of Gram-positive microorganisms in patients with other respiratory infections was 1.18±0.15 Units of OD, of secondary biofilms was 0.35±0.12 Units of OD. The optical density of primary biofilms formed by Gram-negative microorganisms was 2.01±1.03 Units of OD, optical density of secondary biofilms was 1.06±0.42 Units of OD. In patients with pneumonia optical density of primary biofilms of Gram-negative microorganisms was 2.57±0.87 Units of OD, of secondary biofilms was 1.21±0.50 Units of OD. Optical density of primary biofilms of Gram-negative microorganisms in patients with other respiratory infections was 1.24±0.66 Units of OD, of secondary biofilms was 0.84±0.11 Units of OD. Conclusion. Gram-negative microorganisms in general formed more massive biofilms compared with Gram-positive microorganisms. Among all microorganisms P. aeruginosa formed the thickest primary and secondary biofilms. Strains of P. aeruginosa isolated from patients with pneumonia formed the thickest primary and secondary biofilms. Strains of S. aureus isolated from patients with other respiratory infections formed most massive primary biofilms, strains of K. pneumoniae formed the hardest secondary biofilms in this group

Keywords: biofilms, children, respiratory diseases, S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa

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1. Parsek MR, Singh PK. Bacterial biofilms: an emerging link to disease pathogenesis. Annual Review of Microbiology. 2003; 57: 677-701. https://doi.org/10.1146/annurev.micro.57.030502.090720
2. Archer NK, Mazaitis MJ, Costerton JW, Leid J G, Powers ME, Shirtliff ME. Staphylococcus aureus biofilms: properties, regulation, and roles in human disease. Virulence. 2011; 2(5): 445-459. https://doi.org/10.4161/viru.2.5.17724
3. Kiedrowski MR, Horswill AR. New approaches for treating staphylococcal biofilm infections. Annals of New York Academy of Science. 2011; 1241: 104-121. https://doi.org/10.1111/j.1749-6632.2011.06281.x
4. Lister JL, Horswill AR. Staphylococcus aureus biofilms: recent developments in biofilm dispersal. Frontiers in Cellular and Infection Microbiology. 2014; 4(178). https://doi.org/10.3389/fcimb.2014.00178
5. Flemming HC, Wingender J. The biofilm matrix. Nature Reviews Microbiology. 2010; 8: 623-633. https://doi.org/10.1038/nrmicro2415
6. Gunn JS, Bakaletz LO, Wozniak DJ. What’s on the outside matters: the role of the extracellular polymeric substance of gram-negative biofilms in evading host immunity and as a target for therapeutic intervention. Journal of Biological Chemistry. 2016; 291: 12538-12546. https://doi.org/10.1074/jbc.R115.707547
7. Maurice NM, Bedi B, Sadikot RT. Pseudomonas aeruginosa Biofilms: Host Response and Clinical Implications in Lung Infections, American Journal of Respiratory Cell and Molecular Biology. 2018; 58(4): 428-439. https://doi.org/10.1165/rcmb.2017-0321TR
8. Fazli M, Almblad H, Rybtke ML, Givskov M, Eberl L, Tolker-Nielsen T. Regulation of biofilm formation in Pseudomonas and Burkholderia species. Environmental Microbiology.2014; 16(7): 1961-1981. https://doi.org/10.1111/1462-2920.12448
9. Scherr TD, Heim CE, Morrison JM, Kielian T. Hiding in plain sight: interplay between staphylococcal biofilms and host immunity. Frontiers in Immunology. 2014; 5(37). https://doi.org/10.3389/fimmu.2014.00037
10. Boisvert AA, Cheng MP, Sheppard DC, Nguyen D. Microbial Biofilms in Pulmonary and Critical Care Diseases. Annals of the American Thoracic Society. 2016; 13(9): 1615-1623. https://doi.org/10.1513/AnnalsATS.201603-194FR
11. Mulcahy LR, Isabella VM, Lewis K. Pseudomonas aeruginosa biofilms in disease. Microbial Ecology.2014; 68(1): 1-12. https://doi.org/10.1007/s00248-013-0297-x
12. Lebeaux D, Ghigo JM, Beloin C. Biofilm-related infections: bridging the gap between clinical management and fundamental aspects of recalcitrance toward antibiotics. Microbiology and Molecular Biology Reviews. 2014; 78(3): 510-543. https://doi.org/10.1128/MMBR.00013-14
13. Tolker-Nielsen T. Pseudomonas aeruginosa biofilm infections: from molecular biofilm biology to new treatment possibilities. Journal of Pathology, Microbiology and Immunology. 2014; 138: 1-51. https://doi.org/10.1111/apm.12335
14. Alhede M, Bjarnsholt T, Givskov M, Alhede M. Pseudomonas aeruginosa biofilms: mechanisms of immune evasion. Advances in applied microbiology. 2014; 86: 1-40. https://doi.org/10.1016/B978-0-12-800262-9.00001-9
15. Miller JM, Binnicker MJ, Campbell S, Carroll KC, Chapin KC, Gilligan PH, et al. A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2018 Update by the Infectious Diseases Society of America and the American Society for Microbiologya. Clinical Infectious Diseases: an official publication of the Infectious Diseases Society of America. 2018; 67(6): e1-e94. https://doi.org/10.1093/cid/ciy381
16. Romanova Iu M, Alekseeva, NV, Smirnova TA, Andreev AL, Didenko LV, Gintsburg AL. [Biofilms formation by different strains of Salmonella typhimurium in artificial systems]. Zhurnal Microbiologii, Epidemiologii i Immunobiologii. 2006; 4: 38-42.
17. Deinhardt-Emmer S, Haupt KF, Garcia-Moreno M, Geraci J, Forstner C, Pletz M, Ehrhardt C, Löffler B. Staphylococcus aureus Pneumonia: Preceding Influenza Infection Paves the Way for Low-Virulent Strains. Toxins. 2019; 11(12): 734. https://doi.org/10.3390/toxins11120734
18. Al-Wrafy F, Brzozowska E, Górska S, Gamian A. Pathogenic factors of Pseudomonas aeruginosa – the role of biofilm in pathogenicity and as a target for phage therapy. Postepy Higieny i Medycyny Doswiadczalnej (online), 2016; 71(0): 78-91. https://doi.org/10.5604/01.3001.0010.3792
19. Curran CS, Bolig T, Torabi-Parizi P. Mechanisms and Targeted Therapies for Pseudomonas aeruginosa Lung Infection. American Journal of Respiratory and Critical Care Medicine. 2018; 197(6): 708-727. https://doi.org/10.1164/rccm.201705-1043SO
20. Costerton JW. The Biofilm Primer (VIII) Vol 1. Berlin: Springer, 2007. 200 p.
21. Kreft J-U. Biofilms promote altruism. Microbiology. 2004; 150(8): 2751-2760. https://doi.org/10.1099/mic.0.26829-0
22. Mehta P, Goyal S, Long T, Bassler BL, Wingreen NS. Information processing and signal integration in bacterial quorum sensing. Molecular Systems Biology. 2009; 5: 325. https://doi.org/10.1038/msb.2009.79
23. Gostev VV, Sidorenko SV. Bacterial biofilms and infection. Journal of Infectology. 2010; 2(3). https://doi.org/10.22625/2072-6732-2010-2-3-4-15