ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 13 of 57
УЖМБС 2020, 5(1): 91–95
Experimental Medicine and Morphology

Sperm Shaping during the Electromagnetic Field Action

Sharapova O. M.

There are factors that adversely affect the health of workers at the modernized enterprises of the metallurgical, chemical, mining industries of the country. The electromagnetic field is one of the possible harmful factors, which reduces the level of health and as a result, deteriorates the working capacity of workers. The purpose of the study was to determine the effects of the electromagnetic field of high voltage and low frequency on the genitals of male rats, namely, to determine the number of degenerative forms of spermatozoa formed as a result of mitotic and meiotic disorders at the stage of late spermatids. Material and methods. 50 male rats were exposed to a 750 kV electromagnetic field with a frequency of 50 Hz and a power of 10 kV/m at the "Dnepropetrovskaya" electrical substation in the city of Dnepr. The cells were located at a distance of 75 m from the surface of the earth. The control group consisted of 10 rats. Animals were extracted on the 14th, 30th, 45th, 90th, 120th day after the end of the experiment. Histological preparations were made from the blocks, the morphological evaluation of the results of which was carried out on a LEICA CME microscope. Results and discussion. Special attention was paid to the evaluation of germ cell polymorphism, since the degeneration of sperm cells is directly related to their modification, which is the result of aberrations of cell differentiation. The percentage of normal and degenerative forms of spermatozoa was calculated per 100 cells. Pathological processes developed in the testicles of rats in the process of spermatogenesis under the influence of high-voltage electromagnetic field of low frequency, which led to the modification and degeneration of spermatozoa due to impaired mitotic and meiotic divisions. Reproductive function declined in experimental animals. This effect correlates with the “horizontal transfer” of genes, which finds a valid theory in modern evolutionary biology. In the course of the study, it was proved that with an increase in the time of irradiation by the electromagnetic field the number of degenerative cells increased from 20 to 83%, which was confirmed by statistical confidence. Degenerative forms of spermatozoa looked like cells with double centrioles and double flagella, which is explained by an increase in the DNA content in the heads of germ cells and an imbalance in the apparatus of pressure at the level of chromosomes. The action of the electromagnetic field of high voltage and low frequency in the testicles of rats led to edema of the interstitial tissue due to impaired blood circulation of the organ. Interstitial edema entailed a decrease in the average diameter of the testicular tubules from 0.241±0.063 to 0.232±0.081 mm and a decrease in the total area of spermatogenic tissue, which was also statistically confirmed. Conclusion. The obtained results indicated a negative effect of the electromagnetic field on the morphology of the genital organs of male rats in general and on the process of spermatogenesis in particular.

Keywords: spermatogenesis, sperm cell, electromagnetic field, polymorphism

Full text: PDF (Ukr) 210K

  1. Nykytyn NA, Nykytyna AV, Batynger AV. Anatomycheskye osobennosty venoznogo ottoka ot reproduktyvnykh organov krys [Anatomical features of venous outflow from rat reproductive organs]. Byulleten sybyrskoy medytsyny. 2012; 2: 84-92. [Russian]
  2. Jalali М. Study of Spermatogenesis Fetal Testis Exposed Noise Stress During and after Natal Period in rat. Pakistan Journal of Biological Sciences. 2013; 16(19): 1010-5. PMID: 24502163.
  3. Qin F, Zhang J, Cao H, Guo W, Chen L, Shen O, et al. Circadian alterations of reproductive functional markers in male rats exposed to 1800 MHz radiofrequency field. Chronobiol Int. 2014; 31(1): 123-33. PMID: 24117058.
  4. Vereshchako GG. Elektromagnytnoge yzluchenye dyapazona mobylnoy svyazy y ego vlyyanye na muzhskuyu reproduktyvnuyu systemu y potomstvo [Electromagnetic radiation of the mobile range and its effect on the male reproductive system and offspring]. Mynsk: Belarusskaya nauka; 2015. 246 p. [Russian]
  5. Andreeva VM, Gazhat SS, Sorokyna TM. Formy patozoospermyy u muzhchyn s besplodyem v brake y/yly s narushenyem reproduktyvnoy systemy [Forms of pathozoospermia in men with infertility in marriage and / or with a violation of the reproductive system]. Andrologyya y genytalnaya khyrurgyya. 2017; 18(2): 33-4. [Russian]
  6. Bragyna EE, Sorokyna TM. Genetychesky obuslovlennye formy patozoospermyy. Obzor lyteratury y rezultaty yssledovanyy [Genetically determined forms of pathozoospermia. Literature review and research results]. Andrologyya y genytalnaya khyrurgyya. 2015; 16(3): 29-31. [Russian]
  7. Elyseeva YY, Yuzbashev MM. Obshchaya teoryya statystyky [General theory of statistics]. Moskva: Fynansy y statystyka; 2005. 324 p. [Russian]
  8. Khem A, Kormak D. Gystologyya [Histology]. Moskva: Myr; 1983. 294 p. [Russian]
  9. Tkachyshyn VS. Vplyv vyrobnychykh faktoriv na spetsyfichni funktsiyi cholovichogo organizmu [Influence of viral factors on the specific functional functions of the organism]. Medychni perspektyvy. 2017; 3(1): 129-34. [Ukrainian]
  10. Subbotyna TY, Tereshkyna OV, Khadartsev AA. Yzmenenyya v spermatogeneze mlekopytayushchykh pry vozdeystvyy nyzkoyntensyvnogo KVCh-yzluchenyya [Changes in spermatogenesis of mammals when exposed to low-intensity EHF radiation]. Vestnyk novykh medytsynskykh tekhnologyy. 2006; XIII(1): 36-9. [Russian]