ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 52 of 60
Up
УЖМБС 2019, 4(6): 356–361
https://doi.org/10.26693/jmbs04.06.356
Biology

Indices of Lipid Profile of Blood Serum in Young and Old Rats with Alimentary Obesity on the Background of Combined Application of Extreme Cooling and Cord Blood Nucleated Cells

Chernyavskaya E. A. 1, Babijchuk V. G. 1, Sirotenko L. A. 2
Abstract

Alimentary obesity is a chronic disease with a multifactorial nature, being largely stipulated by the lack of motor activity and hypercaloric nutrition. The obesity increases the morbidity and mortality risks because of various complications and requires an interdisciplinary approach in designing therapeutic and preventive methods. In current science of great interest is the study of cold effect on human and animal body. Scientific and clinical practice has accumulated much information on the positive effect of cord blood on both different organs, systems, cell cultures, and the whole body as well. In alimentary obesity, the lipid metabolism is disturbed and an excess fat is accumulated. Proceeding from the mentioned above the purpose of research was to study the lipid profile of blood serum in young and old experimental animals with alimentary obesity in a combined use of rhythmic extreme cold exposures and cryopreserved preparation of cord blood nucleated cells. Material and methods. The research was performed in 6- and 24-month white outbred male rats. All the animals were divided into 3 groups: the first group consisted of intact rats; the second group comprised control rats with the simulated alimentary obesity; the third group was made up of rats with alimentary obesity on the background of combined application of rhythmic extreme cold exposures and cryopreserved preparation of cord blood nucleated cells. The animals were sacrificed by decapitation on the following day. One week and in a month after rhythmic extreme cold exposures and the introduction of cryopreserved preparation of cord blood nucleated cells, the blood was taken and the serum was obtained for biochemical studies. Alimentary obesity was simulated by the method of V. Baranova, by keeping the animals on a hypercaloric diet. Rhythmic extreme cold exposures was performed in cryochamber to cool the experimental animals (–120оC). The thawed preparation of cord blood nucleated cells was intraperitoneally administered once at a dose of 3×105 CD34+ cells per kilogram of body weight. Results and discussion. During an experimental study, it was found out that the development of nutritional obesity led to the appearance of hyperlipidemia and a shift in the lipid profile towards atherogenicity. The level of total cholesterol, low density lipoproteins and the coefficient of atherogenicity increased. It was also noted that these changes in lipid profile were more pronounced in older animals. The combined use of two methods contributed to the normalization of the serum lipid profile of both young and old rats with a model of nutritional obesity, bringing it closer to the indices of control animals without obesity. Atherogenic risk was reduced by lowering the level of total cholesterol, low density lipoproteins and the atherogenic coefficient, as well as increasing the content of high density lipoproteins

Keywords: alimentary obesity, cord blood nucleated cells, rhythmic extreme cold exposures, lipid profile

Full text: PDF (Rus) 215K

References
  1. Razina AA, Runenko SD, Achkasov YeYe. Problema ozhireniya: sovremennyye tendentsii v Rossii i v mire. Vestnik RAMN. 2016; 71(2): 154–9. [Russian] https://doi.org/10.15690/vramn655
  2. Berezina MV, Mikhaleva O, Bardymova TP. Ozhireniye: mekhanizmy razvitiya. Sibirskiy meditsinskiy zhurnal. 2012; 7: 15–8. [Russian]
  3. Luzina YeV, Tomina YeA, Zhilina AA. Ozhireniye i zabolevaniya organov pishchevareniya. Klinicheskaya meditsina. 2013; 6: 63–7. [Russian]
  4. Latysheva VYa, Drobyshevskaya VA. Sovremennyye metody lecheniya bol'nykh izbytochnoye massoy tela i alimentarnym ozhireniyem (obzor literatury). Problemy zdorov'ya i ekologii. 2010; 2(4): 58–65. [Russian]
  5. Vasil'kova TN, Baklayeva TB, Matayev SI, Rybina YuA. Rol' ozhireniya v formirovanii serdechno-sosudistoy patologii. Prakticheskaya meditsina. 2013; 7(76): 117–22. [Russian]
  6. Franssen R, Monajemi H, Stroes E, Kastelein J. Obesity and dyslipidemia. Endocrinol Metab Clin N Am. 2011 Sep; 95(5): 893-902. https://doi.org/10.1016/j.mcna.2011.06.003
  7. Yamamoto Y, Hirose H, Saito I, Tomita M, Taniyama M, Matsubara K, et al. Correlation of the adipocytederived protein adiponectin with insulin resistance index and serum hihgdensity lipoprotein-cholesterol, independent of body mass index, in the Japanese population. Clin Science. 2002; 103(2): 137–42. https://www.ncbi.nlm.nih.gov/pubmed/12149104. https://doi.org/10.1042/cs1030137
  8. Aralova MV, Glukhov AA. Obshchaya i mestnaya Krioterapiya v kompleksnom lechenii ran. Vestnik novykh meditsinskikh tekhnologiy. 2015; 22(2): 111–5. [Russian] https://doi.org/10.12737/11855
  9. Kuznetsov DS, Ivakhnenko DV, Ketovaya YeS, Dorokhov YeV. Vliyaniye total'noy krioterapiyu na dinamiku serdechnogo ritma i arterial'nogo davleniya zdorovogo cheloveka. Zdorov'ye i obrazovaniye. 2017; 19(2): 59–60. [Russian]
  10. Babiychuk VG, Marchenko VS, Babiychuk GA. Kholodovoy stress kak faktor korrektsii funktsional'noy arkhitektoniku gematoentsefalicheskogo bar'yera starykh krys. Problemy kriobiologii i kriomeditsiny. 2012; 22(2): 107–17. [Russian]
  11. Belenkov YuN, Privalova YeV, Chekneva IS. Kletochnaya terapiya v lechenii khronicheskoy serdechnoy nedostatochnosti: vidy primenyayemykh stvolovykh kletok, rezul'taty poslednikh klinicheskikh issledovaniy. Kardiologiya i serdechno-sosudistoy khirurgiya. 2008; 15(5): 4–18. [Russian]
  12. Hariharan N, Sussman MA. Cardiac aging – Getting to the stem of the problem. Journal of molecular and cellular cardiology. 2015; 83: 32–36. https://www.ncbi.nlm.nih.gov/pubmed/25886698. https://www.ncbi.nlm.nih.gov/pmc/articles/4459886. https://doi.org/10.1016/j.yjmcc.2015.04.008
  13. Lee MW, Jang IK, Yoo KH, Sung KW, Koo HH. Stem and progenitor cells in human umbilical cord blood. Int J Hematol. 2010; 92(1): 45–51. https://www.ncbi.nlm.nih.gov/pubmed/20577840. https://doi.org/10.1007/s12185-010-0619-4
  14. Watt S, Contreras M. Stem cell medicine: Umbilical cord blood and its stem cell potential. Semin Fetal Neonat Med. 2005; 209(10): 201–7. https://www.ncbi.nlm.nih.gov/pubmed/15927877. https://doi.org/10.1016/j.siny.2005.02.001
  15. Patent 234999 Ukraine. Sposob vydeleniya yadrosoderzhashchikh kletok kordovoy krovi / Babiychuk LA, Grishchenko VY, Ryazantsev VV (UA); zayavnik i vlasnik patentu Institut problem kriobiologii i kriomeditsiny NAN Ukrainy; zayavl 25.05.2007. [Ukrainian]
  16. Patent 92227 Ukraine. Sposob kriokonservirovaniya yadrosoderzhashchikh kletok kordovoy kroy, v tom chisle stvolovykh gemopoetichsekikh kletok / Babiychuk LA, Grishchenko VY, Gurina TM (UA); zayavnik i vlasnik patentu Institut problem kriobiologii i kriomeditsiny NAN Ukrainy; zayavl 11.10.2010. [Ukrainian]
  17. Baranov VG, Baranov NF, Belovintseva MF. Chuvstvitel'nost' k insulinu, tolerantnost' k glyukoze i insulinovaya aktivnost' krovi v krys s alimentarnym ozhireniyem. Problemy endokrinologii. 1972; 6: 52–8. [Russian]
  18. Patent 40168 Ukraine. Kriokamera dlya eksperimental'nogo okhlazhdeniya laboratornykh zhivotnykh / Babiychuk GA, Kozlov OV, Lomakin II (UA); zayavnik i vlasnik patentu Institut problem kriobiologii i kriomeditsiny NAN Ukrainy; zayavl 25.03.2009. [Ukrainian]
  19. Friedewald W, Levy R, Fredrickson D. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem. 1972; 18(6): 499–502. https://www.ncbi.nlm.nih.gov/pubmed/4337382
  20. Atramentova LA, Utevskaya AN. Statisticheskiye metody v biologii. Gorlovka: «Izdatel'stvo fonar'»; 2008. 248 p. [Russian]