Alimentary obesity is a chronic disease with a multifactorial nature, being largely stipulated by the lack of motor activity and hypercaloric nutrition. The obesity increases the morbidity and mortality risks because of various complications and requires an interdisciplinary approach in designing therapeutic and preventive methods. In current science of great interest is the study of cold effect on human and animal body. Scientific and clinical practice has accumulated much information on the positive effect of cord blood on both different organs, systems, cell cultures, and the whole body as well. In alimentary obesity, the lipid metabolism is disturbed and an excess fat is accumulated. Proceeding from the mentioned above the purpose of research was to study the lipid profile of blood serum in young and old experimental animals with alimentary obesity in a combined use of rhythmic extreme cold exposures and cryopreserved preparation of cord blood nucleated cells. Material and methods. The research was performed in 6- and 24-month white outbred male rats. All the animals were divided into 3 groups: the first group consisted of intact rats; the second group comprised control rats with the simulated alimentary obesity; the third group was made up of rats with alimentary obesity on the background of combined application of rhythmic extreme cold exposures and cryopreserved preparation of cord blood nucleated cells. The animals were sacrificed by decapitation on the following day. One week and in a month after rhythmic extreme cold exposures and the introduction of cryopreserved preparation of cord blood nucleated cells, the blood was taken and the serum was obtained for biochemical studies. Alimentary obesity was simulated by the method of V. Baranova, by keeping the animals on a hypercaloric diet. Rhythmic extreme cold exposures was performed in cryochamber to cool the experimental animals (–120оC). The thawed preparation of cord blood nucleated cells was intraperitoneally administered once at a dose of 3×105 CD34+ cells per kilogram of body weight. Results and discussion. During an experimental study, it was found out that the development of nutritional obesity led to the appearance of hyperlipidemia and a shift in the lipid profile towards atherogenicity. The level of total cholesterol, low density lipoproteins and the coefficient of atherogenicity increased. It was also noted that these changes in lipid profile were more pronounced in older animals. The combined use of two methods contributed to the normalization of the serum lipid profile of both young and old rats with a model of nutritional obesity, bringing it closer to the indices of control animals without obesity. Atherogenic risk was reduced by lowering the level of total cholesterol, low density lipoproteins and the atherogenic coefficient, as well as increasing the content of high density lipoproteins
Keywords: alimentary obesity, cord blood nucleated cells, rhythmic extreme cold exposures, lipid profile
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