ISSN 2415-3060 (print), ISSN 2522-4972 (online)
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УЖМБС 2019, 4(5): 85–89
Experimental Medicine and Morphology

Dynamics of Laboratory Blood Markers of Rats after Implantation of Magnets with Protective ZrN Coating

Starikov V. V., Kutsevliak V. I.

The use of orthodontic structures with magnets for the treatment of malocclusions has several advantages. The most promising magnetic materials for orthodontics are Sm-Co and Nd-Fe-B alloys. An essential disadvantage of magnetic materials is their low corrosion resistance, which can be prevented by applying protective coatings of various types to magnets that lead to passivation of the surface of the magnetic material. The purpose of the study was to evaluate the effects of samples of magnets with protective ZrN coatings and without them on the body of experimental rats after subcutaneous implantation based on laboratory blood test results. Material and methods. The research was conducted on 49 male rats, age of animals was 3-3.5 months, and body weight was 180-250 g. There were 7 rats for 3 terms of study in 2 experimental groups and 1 control group (intact animals). The 1st experimental group rats were implanted with Nd-Fe-B magnet with a protective ZrN coating. The 2nd group rats were implanted with an Nd-Fe-B magnet without a protective coating; the 3rd group rats were intact animals. The amount of red blood cells and leukocytes, hemoglobin and leukocyte contents, as well as glycoproteins, glucose, total protein, urea, ALT and AST activity, total bilirubin and iron were determined in blood of rats on the 7th, 14th and 45th days. Results and discussion. According to the research results, the parameters of the general clinical analysis of blood after implantation with and without protective coatings of magnets did not change, compared with intact animals, indicating no toxic effects of implants on haemocytopoiesis. The content of the iron on the 7th, 14th and 45th days of the experiment in blood of rats implanted with coated magnets did not change. In our opinion, it happened due to the absence of penetration of the iron into the blood, and due to the application of protective ZrN coating to the surface of the implant. In rats with implants without coating, there was a moderate liver parenchyma (increased activity of ALT) and an increase in urea level on the backdrop of the increase in the concentration of iron in the blood on the 7th day. But on the 14th day these indices were normalized, but there was an inflammatory reaction to the implant, which was proved by the elevated blood level of glycoproteins – markers of the inflammation; on the 45th day biochemical blood indices did not differ from those in intact animals. Conclusions. After analyzing the results of insertion of implants with and without the coating, we can say that ZrN protective coating is able to prevent the release of them from the iron and reduce the toxic reaction of animals to the insertion of a magnetic implant.

Keywords: Nd-Fe-B magnets, protective ZrN coating, subcutaneous implantation, rats, toxicity, glycoproteins, iron

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