ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 34 of 49
УЖМБС 2019, 4(4): 218–223
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Prospects for Studying Biological Fluids to Determine the Death Prescription

Mishalov V. D.1, Dunaev O. V.2, Kostenko E. Ya.3, Kozlov S. V.4

The article deals with the possibilities of a new direction in the development of research on biological objects for solving applied problems of forensic medicine. There is a tendency to increase the role and wider use of instrumental methods at the present stage of this kind of research. This, in turn, makes it possible to analyze not only known objects in a dead body, but also new ones that have never been studied before. On the other hand, modern science has quite significant amounts of data. The analysis of information and the search for new phenomena and patterns in such a mass of data requires the application of knowledge and method of analysis that have not recently had application in medicine. Modeling processes and the use of logistic analysis methods are becoming an integral part of modern biomedical research. The article provides a summary analysis of the main directions for deciding on the determination of the length of the post-mortem period both on the basis of forensic science methods that have already proven their effectiveness, and the new ones. There is also a question of possibility of research such a relatively new object for forensic medicine, as biological fluids from the tissues of the corpse. The authors offer an interesting view at the informational utility of such objects. We discuss issues that are associated not only with the study of the chemical composition or other properties of an object such as liquid that accommodates certain components. A completely new look at biological liquid, which may be a multi-level system, is proposed. Although it is linked with the organ or system of organs from where it is extracted, but at the same time, it is a completely independent object for study. This can provide interesting and new information in terms of the nature of the relations within the system. The advantage of such studies for the application of a wide variety of instrumental methods of research is emphasized, since liquids can be studied both in liquid form and after drying. The authors substantiate the general use of logistic analysis methods in order to assess the identified patterns and changes, as well as the use of non-standard versions of statistical methods for classifying objects. Such methods can be methods of mathematical classification.

Keywords: prescription time of death, biological liquid, forensic medicine, classification methods

Full text: PDF (Ukr) 230K

  1. Tsokos M, Byard RW. Post mortem changes - overview. In: Encyclopedia of Forensic and Legal Medicine. 2nd ed. 2016; 4: 10-31.
  2. Buchan MJ, Anderson GS. Time Since Death: A Review of the Current Status of Methods used in the Later Postmortem Interval. Journal of the Canadian Society of Forensic Science. 2001; 34(1): 1-22
  3. Vacchiano G, Luna Maldonado A, Matas Ros M, Di Lorenzo P, Pieri M. The cholesterol levels in median nerve and post-mortem interval evaluation. Forensic Science International. 2016 Jan; 265: 29-33.
  4. Ortmann J, Doberentz E, Madea. Immunohistochemical methods as an aid in estimating the time since death. Forensic science international. 2017; 273: 71-9.
  5. Obukhova LM, Edelev NS, Andriyanova NA, Edelev IS. Opredelenie soderzhaniya mioglobina v krovi v sudebno-meditsinskoy praktike: metodicheskie osobennosti i perspektivy [Determination of maintenance of myoglobin in blood in medico-legal practice: methodical features and prospects]. Sudebno-meditsinskaya ekspertiza. 2016; 4: 57-60. [Russian]
  6. Kutyakov VA. Kontsentratsiya makro- i mikroelementov v biologicheskikh obektakh kak diagnosticheskiy priznak v sudebno-meditsinskoy ekspertnoy praktike [Concentration macro- and microelements in biological objects as a diagnostic sign is in medico-legal expert practice]. Sibirskiy meditsinskiy zhurnal. 2015; 3: 14-8. [Russian]
  7. Kostylyev DV. Sudovo-medychne znachennya imunohlobuliniv u syrovattsi trupnoi krovi pry dyferentsiyniy diahnostytsi prychyn smerti [A medico-legal value of immunoproteins is in the serum of a corpse blood at differential diagnostics of reasons of death]. Abstr. PhDr. (Med.). K; 2003. 20 p. [Ukrainian]
  8. Hui Wang, Jiong Mao, Yingbi Li, Haibo Luo, Jin Wu, Miao Liao, Weibo Liang. 5 miRNA expression analyze in post-mortem interval (PMI) within 48 h. Forensic Science International: Genetics Supplement Series. 2013; 4(Issue 1): e190–1
  9. Young ST, Wells JD, Wells JD, Hobbs GR, Bishop CP. Estimating postmortem interval using RNA degradation and morphological changes in tooth pulp. Forensic Sci Int. 2013 Jun 10; 229(1-3): 163.e1-6.
  10. Bilheux HZ, Cekanova M, Vass AA, Nichols TL, Bilheux JC, Donnell RL, et al. A novel approach to determine post mortem interval using neutron radiograp. Forensic Sci Int. 2015 Jun; 251: 11-21.
  11. Vanchulyak OYa. Diahnostyka davnosti nastannya smerti metodom lazernoho polyarymetrychnoho monitorynhu tkanyn lyudyny [Diagnostics of remoteness of offensive of death by the method of the laser polarimetric monitoring of tissues of man]. Abstr. PhDr. (Med.). K; 2007. 20 p. [Ukrainian]
  12. Harazdyuk MS. Chasovyy monitorynh posmertnykh zmin atofluorestsentsiyi plivok likvoru u vyznachenni davnosti nastannya smerti [The sentinel monitoring of posthumous changes of autofluorestention tapes of neurolymph is in determination of remoteness of offensive of death]. Abstr. PhDr. (Med.). K; 2018. 21 p. [Ukrainian]
  13. Nikiforov YaA. Opredelenie davnosti smerti po izmeneniyu elektricheskogo soprotivleniya pochek i Akhillovykh sukhozhiliy [Determination of remoteness of death on the change of electric resistance of buds and Achilles tendons]. Abstr. Dis. PhDr. (Med.). 2003. 159 p. [Russian]
  14. Vavilov AYu, Chirkov VE, Pozdeev AR, Pleshakova NP. O vozmozhnosti primeneniya metoda izmereniya elektricheskogo soprotivleniya pri issledovanii biologicheskikh sred [About possibility of application of method of measuring of electric resistance at research of biological environments]. Problemy ekspertizy v meditsine. Nauchno-prakticheskiy zhurnal. 2004; 1: 21-3. [Russian]
  15. Fedorova OA. Sudovo-medychne vyznachennya davnosti nastannya smerti za krystalohrafiyeyu tkanyn trupa pid chas yoho hnylnoi transformatsiyi [Medico-legal determination of remoteness of offensive of death is after crystallography of tissues of dead body during his putrid transformation]. Abstr. PhDr. (Med.). K; 2016. 23 p. [Ukrainian]
  16. Nazarova LO. Strukturnye metody issledovaniya sleznoy zhidkosti v diagnostika zlokachestvennykh novoobrazovaniy i vospalitelnykh zabolevaniy organa zreniya [Structural methods of research of tear liquid are in diagnostics of malignant new formations and inflammatory diseases of organ of sight]. Abstr. PhDr. (Med.). М; 2001. 22 p. [Russian]