ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 29 of 49
УЖМБС 2018, 3(1): 156–163
Clinical Medicine

C-159T Polymorphism of CD14 Gene and Citokine Profile at Atopic Dermatite in Adults

Litus O. I., Derkach N. V., Litus V. I.

The aim of the study was to research polymorphism of the C-159T gene of the CD14 receptor gene and the levels of serum cytokines in exogenous and endogenous atopic dermatitis in adults. Materials and methods. The study included 96 adult patients with atopic dermatitis. The control group consisted of 90 healthy volunteers. The polymerase chain reaction with electrophoretic detection was used to analyze the polymorphism of the gene. The content of total IgE and cytokines of TNF-α, IL-2, γ-IF, IL-4, IL-5, IL-10, TFR-β in serum was determined by ELISA. Results and Discussion. It was found that the prevalence of the CC genotype leads to an increased risk of developing exogenous atopic dermatitis, which is associated with a high level of total IgE, IL-5 and low IL-10, TGF-β at a given genotype. At the same time, the reduction in the risk of development of both endogenous and exogenous atopic dermatitis is associated with prevalence in the population of genotypes CT and TT. Reducing the risk of atopic dermatitis in the presence of the T allele can be explained by the low concentration of IL-5 and high IL-10, TGF-β in comparison with the homozygous genotype of the CC. Conclusions. The risk of development of atopic dermatitis is authentically increased (Р = 0,033) at a prevalence allele with polymorphic site CD14 of a receptor. In the presence of a genotype of CC in patients with exogenous atopic dermatitis high levels of the general IgE, IL-5 and low IL-10, TFR-β in comparison with other genotypes are characteristic. Decreasing the risk of developing exogenous atopic dermatitis endogenous is associated with a prevalence in population of genotypes of СT and TT. The risk of developing atopic dermatitis in the presence allele is reduced. The T is characterized by low concentration of IL-5 and high IL-10, TFR-β in comparison with a homozygous genotype of CC. The risk of atopic dermatitis development is increased with the prevalence of allele C and decreased with allele T of the polymorphic region C-159T of the CD14 receptor.

Keywords: C-159T polymorphism, cytokines, atopic dermatitis

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  1. Dharmage SC, Lowe AJ, Matheson MC, Burgess JA, Allen KJ, Abramson MJ. Atopic dermatitis and the atopic march revisited. Allergy. 2014; 69 (1): 17–27.
  2. Pyun BY. Natural history and risk factors of atopic dermatitis in children. Allergy, Asthma & Immunology Research. 2015; 7 (2): 101–5.
  3. Garmhausen D, Hagemann T, Bieber T, Dimitriou I, Fimmers R, Diepgen T, Novak N. Characterization of different courses of atopic dermatitis in adolescent and adult patients. Allergy. 2013; 68 (4): 498–506.
  4. Margolis JS, Abuabara K, Bilker W, Hoffstad O, Margolis DJ. Persistence of mild to moderate atopic dermatitis. JAMA dermatology. 2014; 150 (6): 593–600.
  5. Mortz CG, Andersen KE, Dellgren C, Barington T, Bindslev-Jensen C. Atopic dermatitis from adolescence to adulthood in the TOACS cohort: prevalence, persistence and comorbidities. Allergy. 2015; 70 (7): 836–45.
  6. Strachan DP. Hay fever, hygiene, and household size. BMJ: British Medical Journal. 1989; 299 (6710): 1259–60.
  7. Bach J-F. The protective effect of infections on immune disorders. Journal of Pediatric Gastroenterology and Nutrition. 2005; 40 (Suppl 1): S8.
  8. Matricardi PM, Bonini S High microbial turnover rate preventing atopy: a solution to inconsistencies impinging on the hygiene hypothesis? Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology. 2000; 30 (11): 1506–10.
  9. Okada H, Kuhn C, Feillet H, Bach J-F. The “hygiene hypothesis” for autoimmune and allergic diseases: an update. Clinical and Experimental Immunology. 2010; 160 (1): 1–9.
  10. Kantor R, Silverberg JI. Environmental risk factors and their role in the management of atopic dermatitis. Expert Review of Clinical Immunology. 2017; 13 (1): 15–26.
  11. Baldini M, Lohman I Carla, Halonen M, Erickson RP, Holt PG, Martinez FD. A polymorphism* in the 5′ flanking region of the CD14 gene is associated with circulating soluble CD14 levels and with total serum immunoglobulin E. American journal of respiratory cell and molecular biology. 1999; 20 (5): 976–83.
  12. Zhao L, Bracken MB. Association of CD14-260 (-159) C> T and asthma: a systematic review and meta-analysis. BMC medical genetics. 2011; 12 (1): 93.
  13. Koppelman GH, Reijmerink NE, Stine O Colin,  Timothy DH, Whittaker PA, Meyers DS,  Dirkje P, Eugene RE. Association of a promoter polymorphism of the CD14 gene and atopy. American Journal of Respiratory and Critical Care Medicine. 2001; 163 (4): 965–9.
  14. O’Donnell AR, Toelle BG, Marks GB, Hayden CM, Laing IA, Peat JK, Goldblatt J, Le Souëf PN. Age-specific relationship between CD14 and atopy in a cohort assessed from age 8 to 25 years. American journal of respiratory and critical care medicine. 2004; 169 (5): 615–22.
  15. Bieber T, Vieths S, Broich K. New opportunities and challenges in the assessment of drugs for atopic diseases. Allergy. 2016; 71 (12): 1662–5.
  16. Ostropolets NA, Chopyak VV, Kalyuzhna LD, et al. Unifikovaniy klinichniy protokol pervinnoi, vtorinnoi (spetsializovanoi), tretinnoi (visokospetsializovanoi) medichnoi dopomohi «atopichniy dermatit». Klinichna imunolohiya. Alerholohiya. Infektolohiya. 2016; 97 (8): 37–44. [Ukrainian]
  17. Bisyuk YuA, Belohlazov VA, Dubovoy AI, Znamenskaya LK. C159T polimorfizm hena retseptora CD14 u vzroslykh bolnykh s atopicheskim i neatopicheskim fenotipom bronkhialnoy astmoy v populyatsii Kryma. Imunolohiya ta alerholohiya: nauka i praktika. 2013; 4: 9–15. [Russian]
  18. Ober CA, Tsalenko A, Parry R, Cox NJ. second-generation genomewide screen for asthma-susceptibility alleles in a founder population. American Journal of Human Genetics. 2000; 67 (5): 1154–62.
  19. Litonjua AA, Belanger K, Celedón JC, Milton DK, Bracken MB, Kraft P, Triche EW, Sredl DL, Weiss ST, Leaderer BP, Gold DR. Polymorphisms in the 5′ region of the CD14 gene are associated with eczema in young children. Journal of allergy and clinical immunology. 2005; 115 (5): 1056–62.
  20. Zhang YN, Li YJ, Li H, Zhou H, Shao XJ. Association of CD14 C159T polymorphism with atopic asthma susceptibility in children from southeastern China: a case-control study. Genetics and molecular research: GMR. 2015; 14 (2): 4311–7.
  21. Sengler C, Haider A, Sommerfeld C, Lau S, Baldini M, Martinez F, Wahn U, Nickel R. Evaluation of the CD14 C-159 T polymorphism in the German multicenter allergy study cohort. Clinical & Experimental Allergy. 2003; 33 (2): 166–9.