ISSN 2415-3060 (print), ISSN 2522-4972 (online)
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УЖМБС 2017, 2(5): 64–68
Experimental Medicine and Morphology

Specifics of WGA Lectin Receptor Distrsbutions in Rats in Normal Conditions and after Intranatal Introduction of Female Gormones in Second and Third Periods of Pregnancy

Topolenko Т., Matvieishyna T.

Structural features of WGA lectins’ receptors distribution in rats’ testes after intrauterine introduction of utrogestan were examined. WGA lectin distribution of receptors is as follows: the testicular capsule of intact and control rats at all observation periods, except the period from forty to fifteen to sixty days, has a maximum content of the carbohydrate residues of NAc DGlc and sialic acid. From the forty-fifth to sixty days of life, the content of carbohydrate residues to the lectin receptors of wheat germs in the testicle capsule decreases, which is manifested by the decrease in the intensity of the deposition of the benzindine tag in this structures. Experimental animals of both groups from the first to the thirtieth day showed a decrease in the distribution of WGA + receptors in the capsule of the testicle was observed, which is especially pronounced for the offspring of animals receiving utrozhestan during the third period of pregnancy. By the forty-fifth day of life, the level of WGA receptors distribution to lectin of wheat remains stable, and then again significantly decreases during the third month of life. The basement membrane of the vaginal semen tubes of the intact and control groups of the studied groups of animals, starting from the fifth day of observation, is colored in an intermediate shade between dark brown and brown color, somewhat reducing the degree of coloration during the period of the thirties-sixtieth day after birth and returns to the previous level on the ninety days of life. In the offspring of rats received after the introduction of the urethra during pregnancy, there is a steady low level WGA receptors distribution in the basement membrane of the seminal tubules throughout the observation period, especially at the fourteenth, sixtieth and ninetieth day of life. The cytoplasmic membrane and intracytoplasmic inclusion of mast cells and lymphocytes in intact and control groups of animals from the first to the nineteenth life day will include a moderate distribution density of WGA + -receptors (++ / +). In experimental animals, during the entire observation period, the density of WGA receptors distribution on these structures is low (+/-). In the cytoplasmic membrane of the inbred and control animals fibroblasts, the dynamics of receptor expression for lectin in wheat germs is determined as follows: the WGA + - receptors distribution decreases with an increase in the observation period, which is particularly pronounced on the forty fifth and ninth day of life. In experimental animals of both groups, these indices are lower compared to control (±), but the overall tendency is maintained. Regarding fibrocytes: for intact and control animals, the WGA + receptors distribution on the cytoplasmic fibroblasts decreases from fifth to forty fifth day and then increases until the ninety days of observation. In both experimental rats, the WGA + receptors expression on cytoplasmic membranes of fibrocytes almost completely coincides with those characteristic for fibroblasts in these groups The intensity of the coloration of the basal membrane of the testicular wall in the intact and control groups of the animals under study, from the fourteenth to the sixtieth day inclusive, the intensity of the color rises from ++ to +++, and then sharply decreases to + in the ninety days of life. In experimental animals of both groups there is a tendency to decrease the density of WGA distribution receptor as part of the basement membrane of blood vessels with an increase in the period of observation, especially expressed in the thirtieth and sixtieth day of life. Fibers and intercellular intestinal interstitial testis of intact and control rats from birth up moment to forty-fifth day are coloring approximately with the same intensity in brown color. On the sixtieth day of life, a slight increase in the intensity of the color is determined and then on the ninth day the color of these structures is reduced to a light brown color. In the animals offspring obtained after the introduction of a period of gestation during pregnancy, in contrast to interstitial control, there is a consistently low expression of WGA receptors for almost the entire observation period (±/+).

Keywords: testes, rats, WGA receptors, utrogestan

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