ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 41 of 55
УЖМБС 2022, 7(5): 267–271

Indicators of Prooxidant-Antioxidant System and Protein Metabolism in Rats after the Influence of Weak Electromagnetic Fields

Denysenko S. A., Hoidina V. S., Popova T. M.

The purpose of the work was to study the state of the components of the prooxidant-antioxidant system and indicators of protein metabolism in animals after a long period after exposure to weak electromagnetic fields. Materials and methods. The experiment was carried out on three-month-old white outbred rats. The animals were exposed to low-intensity electromagnetic radiation in the centimeter range for 2 months, 4 hours daily. The study of biochemical parameters of blood serum was carried out 3 months after the end of the experimental exposure. When conducting the research, an emitter in the form of a rectangular horn with a base area of 875 cm was used. Energy radiation was expressed in the power flow density in the area where the experimental animals were located. In the comparison group (n=10), animals were placed in a similar chamber every day, but were not exposed to electromagnetic radiation. Research was conducted in the first half of the day, taking into account circadian rhythms. The analysis of biochemical parameters of blood serum was carried out 3 months after the end of exposure, that is, for rats aged 8 months. Results and discussion. It was revealed that in animals exposed to weak electromagnetic radiation, after a long period after the cancellation of the action, there is an imbalance in the prooxidant-antioxidant system; activation of the non-enzymatic link of antioxidant protection is observed – an increase in the level of s-nitrosothiols against the background of a decrease in the level of lipid peroxidation, which, in addition to a destructive effect on the cell membrane, is a necessary element in maintaining the system of renewal of functionally important component of the lipid layer of cell membranes. The experimentally established dysproteinemia (decrease in the α1 fraction and increase in the α2 fraction of globulins) in animals of the main group may indicate a specific change in the enzymatic spectrum of the synthesis of these proteins in the liver. The activation of antioxidant defense components that we discovered is probably a protective measure aimed at maintaining homeostasis after prolonged exposure to electromagnetic radiation. Conclusion. A decrease in the level of final products of protein metabolism was revealed: creatinine and urea, which suggests a decrease in the rate of protein catabolism. It is concluded that in animals, after a long period after exposure to weak electromagnetic fields of the centimeter range, the level of lipid peroxidation is reduced, specific changes in the indicators of protein metabolism are noted, indicating a decrease in the level of their catabolism. It is suggested that this may be related to a decrease in total muscle mass

Keywords: low-intensity electromagnetic radiation, rats, blood serum

Full text: PDF (Ukr) 252K

  1. Stein S, Udasin IG. Electromagnetic hypersensitivity (EHS, microwave syndrome) - Review of mechanisms. Environ Res. 2020 Jul;186:109445. PMID: 32289567.
  2. Belpomme D, Hardell L, Belyaev I, Burgio E, Carpenter DO. Thermal and non-thermal health effects of low intensity non-ionizing radiation: An international perspective. Environ Pollut. 2018 Nov;242(Pt A):643-658. PMID: 30025338.
  3. Sage C, Carpenter DO. BioInitiative Report: A Rationale for a Biologically- based Public Exposure Standard for Electromagnetic Radiation BioInitiative Working Group. 2012 Dec 31. Available from:
  4. Gruber MJ, Palmquist E, Nordin S. Characteristics of perceived electromagnetic hypersensitivity in the general population. Scand J Psychol. 2018 Aug;59(4):422-427. PMID: 29741795.
  5. Kaszuba-Zwoińska J, Gremba J, Gałdzińska-Calik B, Wójcik-Piotrowicz K, Thor PJ. Electromagnetic field induced biological effects in humans. Przegl Lec. 2015;72(11):636-41. PMID: 27012122
  6. Wang J, Li M, Zhu D, Cao Y. Smartphone Overuse and Visual Impairment in Children and Young Adults: Systematic Review and Meta-Analysis. J Med Internet Res. 2020 Dec 8;22(12):e21923. PMID: 33289673. PMCID: PMC7755532.
  7. Belyaev I, Dean A, Eger H, Hubmann G, Jandrisovits R, Kern M, et al. Guideline 2016 for the prevention, diagnosis and treatment of EMF-related health problems and illnesses. Rev Environ Health. 2016 Sep 1;31(3):363-97. PMID: 27454111.
  8. Yang M, Guo W, Yang C, Tang J, Huang Q, Feng S, et al. Mobile phone use and glioma risk: A systematic review and meta-analysis. PLoS One. 2017 May 4;12(5):e0175136. PMID: 28472042. PMCID: PMC5417432.
  9. Shahin S, Banerjee S, Singh SP, Chaturvedi CM. 2.45 GHz Microwave Radiation Impairs Learning and Spatial Memory via Oxidative/Nitrosative Stress Induced p53-Dependent/Independent Hippocampal Apoptosis: Molecular Basis and Underlying Mechanism. Toxicol Sci. 2015 Dec;148(2):380-99. PMID: 26396154.
  10. Gautam R, Singh KV, Nirala J, Murmu NN, Meena R, Rajamani P. Oxidative stress-mediated alterations on sperm parameters in male Wistar rats exposed to 3G mobile phone radiation. Andrologia. 2019 Apr;51(3):e13201. PMID: 30461041.
  11. Megha K, Deshmukh PS, Banerjee BD, Tripathi AK, Ahmed R, Abegaonkar MP. Low intensity microwave radiation induced oxidative stress, inflammatory response and DNA damage in rat brain. Neurotoxicol. 2015 Dec;51:158-65. PMID: 26511840.
  12. Presman AS. Elektromagnytnye polya y zhyvaya pryroda [Electromagnetic fields and wildlife]. M; «Nauka»: 1968. 288 s. [Russian]
  13. Kamyshnykov VS. Klynycheskye laboratornye testy ot A do Ya y ykh dyagnostycheskye profyly [Clinical laboratory tests from A to Z and their diagnostic profiles]. Spravochnoe posobye. M: «MEDpress-Inform»; 2007. 320 c. [Russian]
  14. Marzinzig M, Nussler AK, Stadler J, Marzinzig E, Barthlen W, Nussler NC, et al. Improved methods to measure and products of titrir oxide in biological fluids: nitrite, nitrate and s-nitrosothiols. Nitric Oxide. 1997 Apr;1(2):177-89. PMID: 9701056.
  15. Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, et al. Oxidative stress, prooxidants, and antioxidants: the interplay. Biomed Res Int. 2014;2014:761264. PMID: 24587990. PMCID: PMC3920909.
  16. Menshchykova EB, Lankyn VZ, Zenkov NK, Bondar IA, Krugovykh NF, Trufakin V. Okyslytelniy stress. Prooksydanty y antyoksydanty [Oxidative stress. Prooxidants and antioxidants]. M: Firma «Slovo»; 2006. 556 s. [Russian]
  17. Singh KV, Gautam R, Meena R, Nirala JP, Jha SK, Rajamani P. Effect of mobile phone radiation on oxidative stress, inflammatory response, and contextual fear memory in Wistar rat. Environ Sci Pollut Res Int. 2020 Jun;27(16):19340-19351. PMID: 32212071.
  18. Catic A. Cellular Metabolism and Aging. Prog Mol Biol Transl Sci. 2018;155:85-107. PMID: 29653684. PMCID: PMC5967871.