ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 30 of 68
УЖМБС 2021, 6(5): 214–219
Clinical Medicine

Results of the Study of Morphological Features and Serotonin Accumulative Function of Platelets in Blood Donors

Muliarchuk O. V., Vydyborets S. V.

Blood transfusion service and its social component – donorship must be the priority areas of the state policy because the results of its work are of paramount importance. The main task of the blood transfusion service is supply of high quality components for blood transfusion therapy. Quality of blood components is compliance of properties and specifications of the blood component supplied to the recipient with the set standards. Strict order of conformance with the approved regulations and procedures is important at all technological states and is a cornerstone of blood transfusion service products quality. All actions, planned and implemented, starting with planning donorship and ending with the finished product manufacturing and storage conditions, are important for ensuring the quality as the final result. The purpose of the study was to determine the content of total, platelet and free serotonin in the plasma of primary and active blood donors for further use of the studied parameters to assess the quality of platelet concentrate. Materials and methods. 160 blood donors (118 men and 42 women) were examined, including 110 active donors (85 men and 25 women) who donated blood regularly at least 3 times a year and 50 primary reserve donors (32 men and 18 women), who donated blood for the first time. Primary reserve donors formed a control group. For the convenience of systematization and reproduction of scientific research results, objectification in comparing research data, all examined active donors, depending on the duration of donor experience and, accordingly, increasing the probability of occurrence of hidden platelet metabolism disorder, were divided into three subgroups: subgroup I – 51 donors (39 men and 12 women), whose donor experience lasted from 2 to 5 years; subgroup II – 31 donors (24 men and 7 women), duration of donor experience which ranged from 6 to 9 years; subgroup III – 28 donors (22 men and 6 women), whose donor experience lasted 10 years or more. The method of fractional determination of biological amines – serotonin – is presented in preliminarily dried biostrate specimens. The method described includes some extraction procedures with optimal controlled pH values necessary for isolating serotonin, producing fluorophors in accordance with orthophtaldialdehide and ninhydrin, the subsequent fluorimetric estimation of their levels being performed on the native fluorometer «БИАН». Results and discussion. It was found that active donors compared to control, have a level of free serotonin in peripheral blood platelets which was significantly higher. Biochemical shifts were revealed in the background of certain morphological changes of platelets. Possible pathophysiological mechanisms of the detected changes are discussed in the article. Conclusion. Active blood donation is accompanied by significant changes in the morphological parameters of platelets and the content of free serotonin in peripheral blood platelets. Further study of platelet hematopoiesis of active blood donors is required

Keywords: blood donation, donors, peripheral blood, platelets, free serotonin

Full text: PDF (Ukr) 261K

  1. AABB Standards for Blood Banks and Transfusion Services. 30th ed. AM ASSN BLOOD; 2016. 120 p.
  2. Blood safety and availability (2020). Available from:
  3. Botos L, Ou DA, Gadlem D, Shallert T, Stenli D, Podolchak N, Volok O, et al. Donorstvo: zalutshennja donoriv krovi ta ii komponentiv [Donation: attraction of donors of blood and its components]. Kyiv-Washington DC; 2014. 200 p. [Ukrainian]
  4. Guide to the preparation, use and quality assurance of blood components: Recommendation No. R(95)15. 19th ed. European directorate for the Quality of medicines & HealthCare: Strasbourg. 2017. 540 p.
  5. Stenly J, Volok A, Gaidukova S, Verbitsky P, Vydyborets S, Kostina M, et al. Menedzhment jakosti v sluzhbi krovi [Quality management in blood services]. Kyiv–Washington DC; 2017. 308 p. [Ukrainian]
  6. Charbonneeau J, Cloutier M-S, Carrier E. Why do blood donors lapse of reduce their donation’s frequency? Social aspect of blood donation, INRS, Montreal, QC, Canada. Transfus Med Rev. 2016; 30: 1-5.
  7. Enitsyan K, Antonova N. Socialnyie i pshichologicheskye factory privlecheniya i uderzhaniya donorov krovi: obzor obzorov [Social and psychological factors of recruitment and retention of blood donors: review of reviews]. Vestnik of Saint Petersburg University. 2019; 9(1): 32-44.
  8. Lunyova GG, Ed. Klinichna biohimiya [Manual of Clinical Biochemistry]. K: Atika; 2013. 1156 p. [Ukrainian]
  9. Mazurov AV. Fisiologiya i patologiya trombocitov [Physiology and Pathology of Platelets]. M: Izdatelstvo Littera; 2011. 480 p. [Russian]
  10. Vynnyk OI, Ed. Dovidnyk z laboratornoji diagnostyki Sinevo [Laboratory diagnostics Synevo: guidlain]. K: Synevo; 2019. 732 p. [Ukrainian]
  11. Vydyborets SV, Gaidukova SM, Muliarchuk OV. Trombocyty: struktura i funkziy [Platelets: structure and function]. Simeyna medyzyna. 2018; 2(76): 103-108. [Ukrainian]
  12. Mykhailychenko BV, Vydyborets SV. Metod odnochasnogo fluorymetrychnogo vyznachennya biogennyh aminiv v analizovaniy probi biosubstratu [The simultaneous fluorimetric assay of biogenic amines in biological specimens]. Laboratorna diagnostyka. 1999; 2(8): 58-61. [Ukrainian]
  13. Rabinovich NL, Makotchenko VM, Kozinets IM. K metodike razdel’nogo opredelenija obschego, trombocytarnogo i svobodnogo serotonina v plasme krovi [The method of separate definition on general, platelet and floating serotonin in blood plasma]. Laboratornoye delo. 1985; 12: 729-731. [Russian]
  14. Yabut JV, Crane JD, Green AE. Keating DG, Khan WI, Steinberg GR. Emerging roles for serotonin in regulating metabolism: New implications for an ancient molecule. Endocrine reviews. 2019; 40(4): 1092-1107.
  15. El-Merahbi R, Loffer M, Mayer A,Sumara G. The roles of peripheral serotonin in methabolic homeostasis. FEBS Letters. 2015; 589(15): 1728-1734.
  16. Oh CM, Namkung J, Go Y, Shong KE, Kim KH, Shong M. Regulation of systemic energy homeostasis by serotonin in adipose tissues. Nature communications. 2015; 6(10): 1-12.
  17. Martin AM, Young RL, Leong L, Rogers GB, Spencer NJ, Jessup CF, Keating DJ. The diverse metabolic roles of peripheral serotonin. Endocrinology. 2017; 158(5): 1049-1063.
  18. Xu L, Cheng D, Huang Z, Ding S, Zhang W, Tan H, et al. Histamine promotes the differentiation of macrophages from CD11b+ myeloid cells and formation of foam cells through a Stat6-dependent pathway. Atherosclerosis. 2017; 263: 42-52.
  19. Mortensen JK, Kranglung KL, Jonsen SP, Mors O, Andersen G, Buttenschon HN. The serotonin transporter gene polymorphisms and risk of isschemic stroke. Cerebrovascular Diseases. 2018; 45(3-4): 187-192.
  20. Belagaje SR. Stroke rehabilitation. Continuum (Minneap Minn). 2017; 23(1): 238-253.