The physicochemical properties (surface tension, dynamic viscosity, crystallization and melting temperatures) of polyvinyl alcohol solutions of molecular weight 9, 31 and 72 kDa have been studied. The surface tension and the critical concentration of micelle formation were determined by the method of stalogometry, and the dynamic viscosity was determined using an Oswald viscometer. The crystallization and melting temperatures were determined in a cooled modified chamber of the UOP-6 software freezer at a rate of 2°C/min. Cryomicroscopic studies were carried out on a polarizing microscope "MIN-8". The surface tension reflects the interaction of PVA solutions with the lipid layer of biomembranes and indicates the hydrophobic properties of substances. The viscosity of PVA solutions characterizes their interaction with water molecules and reflects hydrophilic interactions. The purpose of the study is to determine the physicochemical properties of PVS that characterize the hydrophilic-hydrophobic interactions in the studied solutions and the micelle formation of PVА solutions of different molecular weights. Materials and methods. Studies of the dynamic viscosity and density of 0.1%-1% PVA solutions of molecular weight 9, 31 kDa showed that these parameters increase with increasing PVA concentration, which leads to increased hydrophilicity of the solutions. Results and discussion. It was shown that the surface tension of PVA solutions decreases with increasing concentration, which leads to a decrease in the hydrophobic properties of the polymer. It was found that in 0.5% PVА solutions of molecular weight 9 and 31 kDa the crystallization and melting temperatures decrease from -5 to -6°C. At these temperatures, crystallization and melting of the solutions begin. Conclusion. The study of micelle formation in PVА solutions of different molecular masses was carried out, surface tension isotherms were constructed, and the break point on the isotherm corresponding to the CCM was determined. The values of the critical concentration of micelle formation of PVА of molecular masses 9, 31, 72 kDa were determined. Hydrophobic links of PVА of molecular masses 9 and 31 kDa form hydrophobic cavities in the micelle structure, which can reduce recrystallization activity

Keywords: surface tension, dynamic viscosity, polyvinyl alcohol, melting point, crystallization temperature, micelle formation

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1. Mazur P. Freezing of living cells: Mechanisms and implications. Am J Physiol. 1984; 247: 125–42. https://doi.org/10.1152/ajpcell.1984.247.3.C125
2. Fowler A, Toner M. Cryo-injury and biopreservatio. Ann NY Acad Sci. 2005; 1066: 119–35. https://doi.org/10.1196/annals.1363.010
3. Jin B, Kusanagi K, Ueda M, Seki S, Valdez DM Jr, Edashige K, Kasai M. Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival. Cryobiology. 2008; 56: 233-40. https://doi.org/10.1016/j.cryobiol.2008.03.04
4. Congdon T, Notman R, Gibson MI. Antifreeze (glyco)protein mimetic behavior of poly(vinyl alcohol): detailed structure ice recrystallization inhibition activity study. Biomacromolecules. 2013; 14: 1578–86. https://doi.org/10.1021/bm400217j
5. Deller RC, Vatish M, Mitchell DA, Gibson MI. Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing. Nature Communications. 2014; 5: 3244–51. https://doi.org/10.1038/ncomms4244
6. Gibson MI, Barker CA, Spain SG, Albertin L, Cameron NR. Inhibition of ice crystal growth by synthetic glycopolymers: implications for the rational design of antifreeze glycoprotein mimics. Biomacromolecules. 2009; 10: 328–33. https://doi.org/10.1021/bm801069x
7. Matsumura K, Hyon SH. Polyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties. Biomaterials. 2009; 30: 4842–49. https://doi.org/10.1016/j.biomaterials.2009.05.025
8. Gibson MI. Slowing the growth of ice with synthetic macromolecules: beyond antifreeze (glyco) proteins. Polym Chem. 2010; 1: 1141–52. https://doi.org/10.1039/C0PY00089B
9. Weng L, Swei A, Toner M. Role synthetic antifreeze agents in catalyzing ice nucleation. Cryobiology. 2018; 84: 91-4. https://doi.org/10.1016/j.cryobiol.2018.08.010
10. Dyubko T, Pivovarenko V, Chekanova V, Hvozdiuk Y, Pakhomova Y, Kompaniets A, et al. Study of aggregation of polyvinyl alcohols (9 and 31 kDa) in aqueous solution by fluorescence probing. Biophysical Bulletin. 2020; 44: 5-15 [Ukrainian]
11. Hvozdiuk Yana V, Chekanova Valentina V, Pakhomova Yulia S. Physicochemical properties and cryoprotective action of solutions of polyvinyl alcohol, glycerol and media based on their combinations during freezing of red blood cells. Problems of Cryobiology and Cryomedicine. 2020; 30(3): 288. [Ukrainian]
12. Voyutskiy SS, Panich RM. Praktikum po kolloidnoy khimii i elektronnoy mikroskopii [Workshop on Colloid Chemistry and Electron Microscopy]. M: Chemistry; 1974. 224 p. [Russian]
13. Gorbachev SV. Praktikum po fizicheskoy khimii [Physical chemistry worksop]. M: Higher school; 1974. 496 p. [Russian]
14. Rachinskiy FYu, Rachinskaya MF. Laboratornaya tekhnika [Laboratory technique]. L: Chemistry; 1982. 284 р. [Russian]
15. Damas C, Leprince T, Viet Ngo ThH, Coudert R. Behavior study of polyvinyl alcohol aqueous solution in presence of short chain micelle – forming polyols. Colloid Polymer Science. 2008; 286: 999-007. https://doi.org/10.1007/s00396-008-1862-6
16. Rosenberg ME. Polimery na osnove vinilatsetata [Vinyl acetate based polymers]. L: Chemistry; 1983. 176 p. [Russian]
17. Chen C, Zhy Y, Bao H, Zhao P. Solvent-assisted poly(vinylalcohol) gelated crystalline colloidal array protonic crystals. Soft matter. 2011; 7: 915-21. https://doi.org/10.1039/C0SM00923G
18. Koganovsky AM, Levchenko TM, Kirichenko VA. Adsorbtsiya rastvorennykh veshchestv [Adsorption of solutes]. K: Naykova Dumka; 1977. 130 – 32. [Ukrainian].
19. Choi JH, Kos W. Phase Behavior and Physical Gelation of High molecular weight syndiotactic poly (vinyl alcohol) solution. Macromolecules. 2001; 34: 2964-72.
20. David T, Peggy G. Self-nucleation and crystallization of polyvinyl alcohol. Jornal of Thermal Analysis and Calorimetry. 2017; 127 (1): 885-94.
21. Holmberg K. Surfactants and polymers in aqueous solutions. M: Binom. Knowledge Laboratory; 2007. 528 p.
22. Rusanov AI. Mitselloobrazovanie v rastvorakh poverkhnostno-aktivnykh veshchestv [Micel formation in surfactant solutions]. SPb: Chemistry; 1992. 528 р. [Russian]
23. Serdyuk AI, Kucher RV. Mitsellyarnye perekhody v rastvorakh poverkhnostno-aktivnykh veshchestv [Micellar transitions in a solution of surfactants]. K: Naukova Dumka; 1981. 202 p. [Ukrainian]
24. Chekanova VV, Pakhomova YuS, Kompaniets AM. Mitselloobrazovanie v rastvorakh oksietilirovannykh glitserinov i kombinirovannykh sredakh na ikh osnove [Micelle formation in solutions of ethoxylated glycerols and combined media]. Bulletin of KhNU im VN Karazina. Seriya: Chemistry. 2016; 39-44. [Ukrainian]
25. Chekanova VV, Pakhomova YUS, Dyubko TS, Kompaniets AM. Adsorption activity and micello formation in solutions of oxyethylated glycerins with cryoptective properties during blood cell. Tezice of the conference «Oligomers», 2017. p. 201.