The microbiocenosis of the vagina is a set of microorganisms that inhabit this habitat, normally represented mainly by lactobacilli. The condition of the microbiocenosis of the vagina is of great importance for reproductive health. Bacterial vaginosis and aerobic vaginitis are infectious diseases of the vagina caused by imbalances between physiological and opportunistic microflora, which are normally found in small quantities. The purpose of the study. The work is devoted to the study and analysis of the microflora of the urogenital tract of women in different periods of life with dysbiotic disorders. Materials and methods. A study of the vaginal microflora of 50 women of different ages who complained to a gynecologist was carried out. Microscopic examination of biological material from the vagina using Pappenheim staining showed complete or partial absence of lactobacilli in the samples and their replacement by other bacteria. Smear microscopy showed an increased content of epithelial cells, leukocytes and "key cells" - Gardnerella vaginalis in 25% of women of the 2nd age category (25-35 years old). In women of the 1st and 3rd age categories (15-25 and 45-65 years old, respectively), the content of epithelial cells and leukocytes was normal or slightly higher than normal. Results and discussion. In no case were gonococci and Trichomonas detected in the examined smears. Lactobacilli (bacillus flora) were most often registered in the 1st age category - almost 80%, against 12% in the second and 13% in the third. In 68% of women of the 2nd age category the mixed coco-stick flora prevailed; in the 3rd age group mixed microflora was found in 36% of people; in the first – was absent. Poor microflora was most often found in group 3 of women – 66%, against 20% in group 2, and was not observed in women of group 1. Real-time polymerase chain reaction was used to identify microorganisms in the studied samples. Conclusion. As a result of the survey of women of all ages, 155 strains of pathogenic microorganisms were isolated. The largest number of samples contained Candida spp. (18.7%), Staphylococcus spp. (10.3%), Gardnerella vaginalis (9.7%), Streptococcus spp. (7.1%), Mobiluncus spp., Atopobium vaginae, Leptotrichia spp., Eubacterium spp. were detected in a small number of samples, Ureaplasma urealytic and Mycoplasma hominis were not detected

Keywords: vaginal normocenosis, lactobacilli, real-time polymerase chain reaction, anaerobes, candidiasis, dysbacteriosis

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1. Kafarskaya LI, Korshunova OV, Efimov BA, Volodin NN, Korshunov VM. Mikrobnaya ekolohiya vlagalischa [Microbial ecology of the vagina]. Journal of Mikrobiology, Epidemiology and Immunobiology. 2002; 6: 91-99. [Russian]
2. Andrіets OA. Problemi reproduktivnoho zdorov'ya divchat Bukovini [Problems of reproductive health for children of Bukovini]. Neonatology, surgery and perinatal medicine. 2013; 3(4): 15-18. [Ukrainian]
3. Baranov AA. Nauchnyie napravleniya podprogrammyi «Zdorovyiy rebenok» – prakticheskomu zdravoohraneniyu [Scientific directions of the subprogram "Healthy child" – practical health care]. Russian Pediatric Journal. 2003; 2: 53-54. [Russian]
4. Zhuk SI, Nochvina EA. Sostav mikroflory kishechnika i vlahalishcha u zhenshchin ranneho reproduktivnoho vozrasta na fone dishormonalnykh rasstroystv [Composition of intestinal and vaginal microflora in women of early reproductive age against the background of dyshormonal disorders]. Collection of scientific works of the Association of Obstetricians and Gynecologists of Ukraine. 2006; 2006: 273-276. [Ukrainian]
5. Bumbulienė Ž, Venclavičiūtė K, Ramašauskaite D, Arlauskienė A, Bumbul E, Drąsutienė G. Microbiological findings of vulvovaginitis in prepubertal girls. Postgrad Med J. 2014; 90(1059): 8-12. doi: 10.1136/postgradmedj.2013.131959
6. Rozhneva IL, Tehnika laboratornih robit [Technique of laboratory works]. D; 2014. 16 s. [Ukrainian]
7. Chayka AV, Rutinskaya AV. Kharakteristika mikrobnoho peyzazha vlahalishcha u devochek prepubertatnoho vozrasta v norme i pri vahinalnom disbioze [Characteristics of the microbial landscape of the vagina in prepubertal girls in normal conditions and with vaginal dysbiosis]. Tavricheskiy medico-biological herald. 2012; 2(58): 204-207. [Russian]
8. Donnikov AE, Vitvitskaya UG, Kan NE, Tyutyunnik VL, Priputneviсh TV, Nikitina IV, et al. Diagnostika infektsiy v akushersko-ginekologicheskoy i neonatologicheskoy praktike [Diagnosis of infections in obstetric-gynecological and neonatological practice]. M; 2018. p. 3-41. [Russian]
9. Vinogradova KO, Gavrilyuk VG, Sklyar TV, Sokolova IV. Monitorinh viyavlennya disbiotichnikh porushen v urohenitalnomu trakti zhinok ta cholovikiv riznikh vikovikh katehoriy [Monitoring of the development of dysbiotic lesions in the urogenital tract of women and men in different categories]. Bulletin of problems of biology and medicine. 2020; 2(156): 226-231. [Ukrainian]
10. Vinogradova KO, Gavrilyuk VG, Sklyar TV, Sokolova IV. Chastota viyavlennya antibiotikorezistentnikh variantiv mikroorhanizmiv-zbudnikiv infektsiynikh protsesiv v okremikh biotopakh urohenitalnogo traktu patsiyentiv riznoho viku i stati [The frequency of detection of antibiotic-resistant variants of microorganisms-diseases in infectious processes in the vicinity of biotopes of the urogenital tract of patients in the growth and statics]. Ukrainian Journal of Medicine, Biology and Sport. 2020; 4(26): 229-239. [Ukrainian] https://doi.org/10.26693/jmbs05.04.229
11. Gospod VV, Golodok LP, Sklyar TV. Osoblivosti skladu mikroflori sechostatevogo traktu u zhinok v normi ta patohenezi [The peculiarities of the microflora of the sechostatic tract in females in the norm and pathogenesis]. Bulletin of problems of biology and medicine. 2020; 4(158): 247-253. [Ukrainian]
12. Trigub SA, Golodok LP, Sklyar TV. Osoblivosti mikrobiotsenozu sechostatevoi sistemi zhinok pri patologichnikh stanakh [Features of microbiocenosis of the urogenital system of women are at pathosiss]. Bulletin of problems of biology and medicine. 2019; 1(148): 189-193. [Ukrainian]
13. Tai FW, Chang CY, Chiang JH, Lin WC, Wan L. Association of pelvic inflammatory disease with risk of endometriosis of nationwide cohort study involving 141,460 individuals. Journal of clinical medicine. 2018; 7(11): 379. PMid: 30352985. PMCid: PMC6262473. https://doi.org/10.3390/jcm7110379