ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 54 of 67
УЖМБС 2020, 5(4): 395–400

Physical Training of White Mice during Swimming in Conditions of Stimulation and Inhibition of Interleukin-2

Shvets V. A., Hasiuk O. M.

Assessment of physical training is currently relevant, as it is known that the physical activity directly affects the cells of the immune system. Despite the sufficient quantity of studies on certain aspects of using IL-2 and its inhibitor, now there is a question about their dose-dependent effect on physical endurance. The purpose of the work was to determine the physical training of white mice in conditions of long-term administration of various doses of recombinant IL-2 and its inhibitor cyclosporine. Material and methods. 5 experimental groups of mice were formed (n=90). Group I received an inhibitor of IL-2 (10 mg/kg), II, III and IV experimental groups got IL-2 (5000, 7500 and 30000 IU/kg, respectively), group V received saline solution. Тhe method of forced swimming was used аs physical training to complete exhaustion with a load (7,5 % of body weight). The physical endurance was measured by the duration of swimming of mice from the moment of entry into the water until complete exhaustion. The study was divided into periods to determine adaptive changes (control, 1-4 and 6 week). Results and discussion. The animals with IL-2 inhibition throughout the experiment the swimming time increased: a significant veracious increase on the 2nd week was by 63±3 %; on the 4th week it increased by 44.4±2 %; on the 6th week it increased by 32±1.5 %. The swimming time of mice with exposure to IL-2 in the average concentration also increased throughout the training period, the largest veracious significant increase was on the 2nd (58.9±3 %), on the 3rd (20.9±1 %) and on the 6th (29.6±1.4 %) weeks. The studied indicator of the group of animals without the introduction of drugs reached the maximum on the 3rd week, then decreased slightly, but remained much higher than the initial, which can be considered the result of training. During the experiment, it was found out that in all experimental animals, the time of forced swimming during 6 weeks significantly exceeded the values obtained after the first day of the training. The maximum increase in physical training on the 6th week (compared to baseline) was observed in the group administered the IL-2 inhibitor (an increase at 267.4±13 %). Animals exposed to medium and high concentrations of IL-2 also showed a veracious increase of the studied indicator (at 161.65±8 % and 85.1±4 %, respectively). Conclusion. The obtained results showed that the inhibition of IL-2 had a positive effect on adaptation to physical training throughout the experiment and caused the most significant increase at the period of forced swimming compared to baseline. The introduction of IL-2 caused an increase in physical training of animals. However, only under the influence of IL-2 in the average concentration (7500 IU/kg), the swimming time increased in the post-action period, which indicated the adaptive effect of IL-2 in this concentration (which can be considered optimal).

Keywords: physical training, forced swimming, interleukin-2, cyclosporine

Full text: PDF (Ukr) 383K

  1. Karkishchenko NN, Redaktor. Biomeditsinskoe (doklinicheskoe) izuchenie lekarstvenykh sredstv, vliyanie na fizicheskuyu rabotosposobnost [Biomedical (preclinical) study of drugs, the impact on physical performance]. Metodichesskie rekomendatsii. M: FMBA Rossii; 2017. 134 p. [Russian]
  2. Kozlov VA, Kudaeva OT. Immunnaya sistema i fizicheskie nagruzki [The immune system and physical activity]. Meditsinskaya immunologiya. 2002; 4(3): 427-38. [Russian]
  3. Nieman DC, Wentz LM. The compelling link between physical activity and the body's defense system. The Journal of Sport and Health Science. 2019; 8(3): 201-17.
  4. Gasyuk OM Samoylenko YuS, Polovynko TO, Leonenko SYu. Fizychna pratsezdatnist v umovakh vplyvu erytropoez-stymulyuyuchogo faktoru [Physical performance under the influence of erythropoiesis-stimulating factor]. Pryrodnychyy almanakh. Biologichni nauky. 2016; 23: 5-13. [Ukrainian]
  5. Peake JM, Della Gatta P, Suzuki K, Nieman DC. Cytokine expression and secretion by skeletal muscle cells: regulatory mechanisms and exercise effects. Exercise immunology review. 2015; 21: 8-25.
  6. Pedersen BK, Hoffman-Goetz L. Exercise and the immune system: regulation, integration, and adaptation. Physiological Reviews. 2000; 80(3): 1055-81. ttps://
  7. Gersner R, Gordon-Kiwkowitz M, Zangen A. Automated behavioral analysis of limbs' activity in the forced swim test. Journal of Neuroscience Methods. 2009; 180: 82-6.
  8. Castagne V, Moser P, Roux S, Porsolt RD. Rodent models of depression: forced swim and tail suspension behavioral despair tests in rats and mice. Current Protocols in Neuroscience. 2011; 8(8).
  9. Okovityi SV, Radko SV. Vliyanie razlichnykh farmakologicheskikh veshchestv na vosstanovlenie fizicheskoy rabotosposobnosti posle nagruzok v eksperimente [The effect of various pharmacological substances on the restoration of physical performance after exercise loads]. Eksperimentalnaya i klinicheskaya farmakologiya. 2018; 81(4): 28-34. [Russian]
  10. Korytko ZI. Suchasni uyavlennya pro zahalni mekhanizmy adaptatsii orhanizmu do dii ekstremalnykh vplyviv [Modern ideas about the general mechanisms of organism adaptation to experimental effects]. Visnyk problem biolohii i medytsyny. 2013; 4(1): 28-35. [Ukrainian]
  11. Sarapultsev PA, Sarapultsev AP. Stress i immunnaya Sistema [Stress and the immune system]. Tsitokiny i vospalenie. 2014; 13(4): 5-10. [Russian]
  12. Hoffmann C, Weigert C. Skeletal Muscle as an Endocrine Organ: The Role of Myokines in Exercise Adaptations. Cold Spring Harbor Perspectives in Medicine. 2017; 7(11).
  13. Pedersen BK, Bruunsgaard H, Ostrowski K, Krabbe K, Hansen H, Krzywkowski K, et al. Cytokines in aging and exercise. International Journal of Sports Medicine. 2000; 21(1): 4-9.
  14. Akerstrom T, Steensberg A, Keller P, Keller C, Penkowa M, Pedersen BK. Exercise induces interleukin-8 expression in human skeletal muscle. The Journal of Physiology. 2005; 563(2): 507-16.
  15. Kapilevich LV, Kabachkova AV, Zakharova AN, Lalaeva GS, Kironenko TA, Dyakova EY, et al. Secretory function of skeletal muscles: producing mechanisms and myokines physiological effects. Uspekhi fiziologicheskikh nauk. 2016; 47(2): 7-26.
  16. Duzova H. Skeletal muscle, myokines and health. Medicine Science. 2012; 1(3): 211-31.
  17. Palanki MS, Manning AM. Interleukin-2 inhibitors in autoimmune disease. Expert Opinion on Therapeutic Patents. 1999; 9(1): 27-39.
  18. Pedersen BK, Akerstrom ThCA, Nielsen AR, Fischer ChP. Role of myokines in exercise and metabolism. The Journal of Applied Physiology. 2007; 103: 1093-8.
  19. Futornyi SM, Imas YeV, Osadcha OI, Shmatova EA, Hlukhovskyi PV. Osoblyvosti imunolohichnoi adaptatsii pid vplyvom znachnykh fizychnykh navantazhen [Features of immunological adaptation under the influence of significant physical exertion]. Naukovyi chasopys NPU imeni MP Drahomanova. 2018; 10(104): 93-8. [Ukrainian]
  20. Tang Q. Therapeutic Window of Interleukin-2 for Autoimmune Diseases. Diabetes. 2015; 64: 1912-3.
  21. Abbas AK, Trotta ER, Simeonov D, Marson A, Bluestone JA. Revisiting IL-2: Biology and therapeutic prospects. Science Immunology. 2018; 3(25).
  22. Ko MH, Chang CK, Wu CL, Hou YC, Hong W, Fang SH. The interactive effect of exercise and immunosuppressant cyclosporin A on immune function in mice. Journal of Sports Sciences. 2010; 28(9): 967-73.
  23. Porsolt RD, Anton G, Blavet N, Jalfre M. Behavioral despair in rats: a new model sensitive to antidepressant treatment. The European Journal of Pharmacology. 1978; 47(4): 379-91.
  24. Moiseev AN, Stepanov AV, Tsikarishvili GV. Ronkoleykin i vozmozhnye mekhanizmy ego vliyaniya na rabotosposobnost zhivotnykh [Ronkoleukin and possible mechanisms of its influence on animal performance]. Aktualnye voprosy veterinarnoy biologii. 2009; 4(4): 19-23. [Russian]