ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 18 of 67
УЖМБС 2020, 5(3): 145–150
Experimental Medicine and Morphology

Dynamics of Indicators of Lipid Metabolism in Rats at Dark Deprivation in Experiment

Sobolevskaya I. S., Myadelets O. D., Yarotskaya N. N.

The purpose of the study was to research the dynamics of lipid metabolism in white male rats during dark deprivation. Material and methods. 40 white outbred male rats with a body weight of 170-220 grams were used in the experiments. The experimental animals were divided into 2 groups: animals under standard fixed lighting conditions (12 hours light / 12 hours darkness); animals with modeling of dark deprivation in the conditions of round-the-clock lighting (24 hours light). Serum concentrations of cholesterol, triaciolglycerols, low-density lipoproteins, high-density lipoproteins, total phospholipids were determined. Results and discussion. On the 7th day of dark deprivation in rats, changes in the lipid composition of blood serum were noted due to 1.61 times increase in the concentration of triacylglycerols and 1.94 times increase in total phospholipids. No changes in the concentration of total cholesterol and its fractions were detected. On the 14th day of exposure to constant light, animals showed a sharp increase in the concentrations of total cholesterol by 1.23 times and low-density lipoproteins by 2.18 times, while the concentration of high-density lipoproteins was not statistically different from that of the intact group. The concentration of total phospholipids remained 1.42 times higher compared with intact animals. On the 21st day of desynchronosis modeling, the changes affected all studied lipid metabolism parameters (total cholesterol concentrations increased by 1.33 times, high-density lipoproteins increased by 1.3 times, low-density lipoproteins were 1.18 times higher and triacylglycerols increased by 1.35 times compared with the control group). Conclusion. Prolonged dark deprivation leads to a violation of the lipid profile of rat’s serum (dyslipoproteinemia). Long-term dark deprivation leads to dyslipoproteinemia, which consists in the increase in total cholesterol concentrations (by 1.33 times), triacylglycerols concentration (by 1.62 times), low-density lipoproteins (by 1.2 times), total phospholipids (by 1.15 times). The severity of changes in the concentration of total cholesterol, triacylglycerols, low-density lipoproteins, high-density lipoproteins and total phospholipids increases depending on the duration of the experiment.

Keywords: dark deprivation, desynchronosis, lipids, serum, rat

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  1. Ramsey KM, Marcheva B, Kohsaka A, Bass J. The clockwork of metabolism. Ann Rev Nutr. 2007; 27: 219-40.
  2. Straif K, Baan R, Grosse Y, Secretan B, El Ghissassi F, Bouvard V, et al. Carcinogenicity of shift-work, painting, and fire-fighting. Lancet Oncol. 2007; 8: 1065-6.
  3. Gnocchi D, Pedrelli M, Hurt-Camejo E, Parini P. Lipids around the Clock: Focus on Circadian Rhythms and Lipid Metabolism. Biology (Basel). 2015; 4(1): 104-32.
  4. Gooley J. Circadian regulation of lipid metabolism. Proceedings of the Nutrition Society. 2016; 75(4): 440-50.
  5. Andersen ML, Perry JC, Bignotto M, Tufik S. Differential effects of sleep loss and chronic stressors on lipid metabolism. Sleep Sci. 2009; 2(3): 135-40.
  6. Pan X, Hussain MM Clock is important for food and circadian regulation of macronutrient absorption in mice. J Lipid Res. 2009; 50: 1800-13.
  7. Hussain MM, Pan X. Circadian regulators of intestinal lipid absorption. J Lipid Res. 2015; 56(4):761-70.
  8. Pan X, Hussain MM. Diurnal regulation of microsomal triglyceride transfer protein and plasma lipid levels. J Biol Chem. 2007; 282(34): 24707-19.
  9. Bae SA, Fang MZ, Rustgi V, Zarbl H, Androulakis IP. At the Interface of Lifestyle, Behavior, and Circadian Rhythms: Metabolic Implications. Front Nutr. 2019; 6: 132.
  10. Javeed N, Matveyenko AV. Circadian Etiology of Type 2 Diabetes Mellitus. Physiology (Bethesda). 2018; 33(2): 138-50.
  11. Bilu C, Zimmet P, Vishnevskia-Dai V, Einat H, Agam G, Grossman E, et al. Diurnality, Type 2 Diabetes, and Depressive-Like Behavior. Journal of Biological Rhythms. 2019; 34(1): 69-83.
  12. Crnko S, Du Pré BC, Sluijter JPG. Circadian rhythms and the molecular clock in cardiovascular biology and disease. Nat Rev Cardiol. 2019; 16: 437-47.
  13. Sotak M, Polidarova L, Musilkova J, Hock M, Sumova A, Pacha AJP. Circadian regulation of electrolyte absorption in the rat colon. Gastrointestinal and Liver Physiology. 2011; 301: G1066-G1074.
  14. Anisimov IG, Popovich MA, Zabezhinski VN. Melatonin as antioxidant, geroprotector and anticarcinogen. Biochim Biophys Acta. 2006; 1757: 573- 89.
  15. Keskin E, Uluişik D. The Protective Effect of Melatonin on Plasma Lipid Profile in Rats with Cerulein-induced Acute Pancreatitis. Turkish Journal of Sport and Exercise. 2019; 21 (2): 332-6.
  16. Shabani A, Foroozanfard F, Kavossian E, Aghadavod E, Ostadmohammadi, V, Reiter RJ, et al. Effects of melatonin administration on mental health parameters, metabolic and genetic profiles in women with polycystic ovary syndrome: A randomized, double-blind, placebo-controlled trial. Journal of Affective Disorders. 2019; 250: 51-6.
  17. Parandavar N, Hojat M, Abdali K, Keshtgar S, Emamghoreishi M, Yeganeh BS. The effect of melatonin on the lipid levels in menopausal women: A double-blind, controlled, clinical trial. J Educ Health Promot. 2018; 7: 144.
  18. Bahrami M, Cheraghpour M, Jafarirad S, Alavinejad P, Cheraghian B. The role of melatonin supplement in metabolic syndrome: A randomized double blind clinical trial. Nutrition & Food Science. 2019; 49(5): 965-77.
  19. Reiter RJ, Tan DX, Maldonado MD Melatonin as an antioxidant: physiology versus pharmacology. Journal of Pineal Research. 2005; 39: 215-6.