The purpose of the study was to research the dynamics of lipid metabolism in white male rats during dark deprivation. Material and methods. 40 white outbred male rats with a body weight of 170-220 grams were used in the experiments. The experimental animals were divided into 2 groups: animals under standard fixed lighting conditions (12 hours light / 12 hours darkness); animals with modeling of dark deprivation in the conditions of round-the-clock lighting (24 hours light). Serum concentrations of cholesterol, triaciolglycerols, low-density lipoproteins, high-density lipoproteins, total phospholipids were determined. Results and discussion. On the 7th day of dark deprivation in rats, changes in the lipid composition of blood serum were noted due to 1.61 times increase in the concentration of triacylglycerols and 1.94 times increase in total phospholipids. No changes in the concentration of total cholesterol and its fractions were detected. On the 14th day of exposure to constant light, animals showed a sharp increase in the concentrations of total cholesterol by 1.23 times and low-density lipoproteins by 2.18 times, while the concentration of high-density lipoproteins was not statistically different from that of the intact group. The concentration of total phospholipids remained 1.42 times higher compared with intact animals. On the 21st day of desynchronosis modeling, the changes affected all studied lipid metabolism parameters (total cholesterol concentrations increased by 1.33 times, high-density lipoproteins increased by 1.3 times, low-density lipoproteins were 1.18 times higher and triacylglycerols increased by 1.35 times compared with the control group). Conclusion. Prolonged dark deprivation leads to a violation of the lipid profile of rat’s serum (dyslipoproteinemia). Long-term dark deprivation leads to dyslipoproteinemia, which consists in the increase in total cholesterol concentrations (by 1.33 times), triacylglycerols concentration (by 1.62 times), low-density lipoproteins (by 1.2 times), total phospholipids (by 1.15 times). The severity of changes in the concentration of total cholesterol, triacylglycerols, low-density lipoproteins, high-density lipoproteins and total phospholipids increases depending on the duration of the experiment.

Keywords: dark deprivation, desynchronosis, lipids, serum, rat

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