The article deals with studying the features of tooth eruption in 12- and 40-day-old rats born with experimentally modeled fetal macrosomia. The investigation takes into account the particularities of animal intrauterine somatotype: macrosomia with acceleration, macrosomia with harmonious (well-balanced) intrauterine development, and relative intrauterine insufficiency of body weight or macrosomia with intrauterine obesity. The purpose of the study was to reveal the features of tooth morphogenesis in 12- and 40-day-old rats born with experimentally modeled macrosomia. Material and methods. This study was conducted using rats of the Wistar Albino Glaxo population. The offsprings of such rats were removed from the experiment on the twelfth and fortieth day after birth. Results and discussion. Macrosomia was modeled using four different macrosomia formation models. Immediately after birth, the rats were weighed and divided into groups, taking into account the calculated weight-height parameters and the modeling of macrosomia. Five groups of animals were formed with 5-6 individuals in each group. The second somatometric examination of the rats (weighing, measurement of body length, and tail length) was conducted immediately before withdrawing animals from the experiment. The lengths of the crowns of the incisors (12-day-old animals) and molars (40-day-old animals) were measured using the MBS-9 stereo magnifier. Enamel demineralization sites and carious cavities were detected by drying and staining with methylene blue. The photofixation of the preparations was carried out with scaled pointers. Conclusion. High birth weight can be an integral indicator of the presence of various features of the morphogenesis of teeth and tissues that surround them. The dominance of pre-natal obesity or pre-natal accelerated body growth, or the well-balanced acceleration of body weight and height gain, has its own characteristics in the tooth development in ontogenesis. The macrosomic-at-birth twelve-day-old rats born with a large body length and a relatively low weight showed a delay in the eruption of incisors. The macrosomic-at-birth 40-day-old rats did not show signs of delayed eruption of the molars, but in macrosomic-at-birth animals with signs of intrauterine obesity the length of the molars tends to decrease. At the age of 40 days, the rats born macrosomic had demineralization of the enamel of the chewing group of teeth. The highest intensity of the carious process is recorded in macrosomic-at-birth animals born with signs of intrauterine obesity.

Keywords: fetal macrosomia, rat, tooth eruption

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1. Garmash OV. An eruption pattern of deciduous teeth in children born with fetal macrosomia during the first year of life. Georgian medical news. 2017; 2(263): 14-23.
2. Goncharova EI. Rost i razvitiye zubov, ikh gormonalnaya regulyatsiya. [The growth and development of teeth, their hormonal regulation]. Rossiyskiy stomatologicheskiy zhurnal. 2013; 1: 53-6. [Russian]
3. Gubina-Vakulyk G. Patologichna anatomiia endokrynnyh zaloz plodu pry adaptatsii vagitnoi do dii okremyh seredovyshchnyh faktoriv [Pathological anatomy of the fetal endocrine glands in pregnant woman adaptation to the some environmental factors action]. Abstr. Dr. Sci. (Med.). Kharkiv National Medical University; 1994. [Ukrainian]
4. Karmakar R. Forensic medicine and toxicology: theory, oral & practical. Academic Publishers (5th ed.). 2015. 612 p.
5. Garmash O. Dependence of Deciduous Tooth Eruption Terms and Tooth Growth Rate on the Weight-Height Index at Birth in Macrosomic Children over the First Year of Life. Acta medica (Hradec Kralove). 2019; 62(2): 62-8. PMID: 31184300. https://doi.org/10.14712/18059694.2019.48
6. Khuraseva AB. Adaptation of newborns and their development in the first year of life depending on body mass at the birth. Nauchnye vedomosty Bel GU. Serya Medycyna. Farmacyya. 2014; 4(175): 102–5. [Russian]
7. Garmash OV, Gubina-Vakulyk GI. Morfofunktsionalnyi stan pryvushnykh slynnykh zaloz trymisiachnykh shchuriv pry eksperymentalno modelovanii vnutrishnoutrobnii makrosomii [Morphofunctional status of parotid salivary glands in three-month-old ratswith experimentally induced fetal macrosomia]. Patolohiia. 2018; 15(1): 81-7. [Ukrainian]
8. Sarkisian YeG. Forma i mikroskopicheskoye stroyeniye korennykh zubov krolika i krysy [Shape and microscopic structure of molars in rabbits and rats]. VISNYK VDNZU «Ukrainska medychna stomatolohichna akademiia». 2015; 2(50): 198-202. [Russian]
9. Addison WHF, Appleton JL. The Structure and Growth of the Incisor Teeth of the Albino Rat. J Morph. 1915; 26: 43-96. https://doi.org/10.1002/jmor.1050260103
10. Grischenko VI, Yakovtsova AF. Krupnyiy plod (kliniko-morfologicheskoe issledovanie) [Large fetus (clinical and morphological study)]. In: VI Grischenko, AF Yakovtsova. K: Zdorovya; 1991. 183 p. [Russian]
11. Poumpros E, Loberg E, Engström C. Thyroid function and root resorption. Angle Orthod. 1994; 64(5): 389-93.
12. Novik IO, Kerimov EE. Vliyaniye gipertireoza na rost, razvitiye i mineralnyy obmen v zubakh [The effect of hyperthyroidism on the growth, development and mineral metabolism in teeth]. Yubileynyy sbornik uchenykh zapisok Azerbaydzhan gos med in-ta. Baku. 1967; 26–27: 383–9. [Russian]
13. Vucic S, Korevaar TIM, Dhamo B, Jaddoe VWV, Peeters RP, Wolvius EB, et al. Thyroid Function during Early Life and Dental Development. J Dent Res. 2017 Aug; 96(9): 1020-6. PMID: 28489513. https://doi.org/10.1177/0022034517708551
14. Savelyeva AYu. Praktikum po anatomii dekorativnykh i ekzoticheskikh zhivotnykh [Workshop on the anatomy of decorative and exotic animals] Krasnoyar gos agrar un-t. Krasnoyarsk; 2018. 284 p. [Russian] [Internet]. Available from: http://docplayer.ru/108700953-A-yu-saveleva-praktikum-po-anatomii-dekorativnyh-i-ekzoticheskih-zhivotnyh.html
15. Loevy HT, Aduss H, Rosenthal IM. Tooth eruption and craniofacial development in congenital hypothyroidism: report of case. J Am Dent Assoc. 1987 Sep; 115(3): 429-31. PMID: 3476664. https://doi.org/10.14219/jada.archive.1987.0254
16. Schour I, Massler M. The teeth. In: The Rat in Laboratory Investigation. 2nd ed. JQ Griffith, EJ Farris, Eds. Philadelphia-London-Montreal; JB Lipincott; 1934. p. 104-65.
17. Must A, Phillips SM, Tybor DJ, Lividini K, Hayes C. The association between childhood obesity and tooth eruption. Obesity (Silver Spring). 2012 Oct; 20(10): 2070-4. PMID: 22310231. PMCID: PMC3574556. https://doi.org/10.1038/oby.2012.23
18. Wong HM, Peng SM, Yang Y, King NM, McGrath CPJ. Tooth eruption and obesity in 12-year-old children. Dent Sci. 2017 Jun; 12(2): 126–32. PMID: 30895037. PMCID: PMC6395248. https://doi.org/10.1016/j.jds.2016.10.004
19. Silvane ES; Vasconcelos KRF, Thaís AX, Oliveira S, Dutra ALT, Nelson-Filho P, et al. Timing of Permanent Tooth Emergence is Associated with Overweight/Obesity in Children from the Amazon Region. Braz Dent J. 2018; 29(5): 465-8. PMID: 30517445. https://doi.org/10.1590/0103-6440201802230
20. Sabharwal R, Sengupta S, Sharma B, Singh S, Rastogi V. Correlation of body mass index with eruption time of permanent first molars and incisors and caries occurrence: A cross-sectional study in school children in Uttar Pardesh, India. Eur J Gen Dent. 2013; 2(2): 114-8. https://doi.org/10.4103/2278-9626.112306