ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 55 of 60
УЖМБС 2019, 4(6): 377–383
Physical training and Sport. Medical and biological aspects of training athletes

Correction of the Hematological Homeostasis Parameters during Physical Loads by Using Energy Orientation Pharmacological Agents

Kuzmenko M. V. 1, Gunina L. M. 2, Nosach O. V. 3, Golovashchenko R. V. 4

The article highlights the influence of the domestic metabolite ATP-long drug containing the main energy phosphate molecule in a protected form on the indicators of the red link of hematological homeostasis, including red blood cell indices, and the parameters of prooxidant-antioxidant equilibrium in erythrocyte membranes, which is of considerable interest in connection with the influence of these characteristics on the oxygen-transport function of the blood, and therefore aerobic performance during intense physical exertion. The choice of the drug was due to the fact that the energy supply of an erythrocyte, which does not have its own energy-generating system and significantly affects its functional characteristics. On the other hand, it provokes structural changes in the erythrocyte membrane, its further deformation and size changes, which makes it difficult for the cells to move along the microcirculation bed and, as a result, disrupts the ability of the oxygen transport system of the blood, thereby impairing the supply of energy and plastic substrates to the myocardium and the athlete’s skeletal muscle. Material and methods. The study was conducted in 25 athletes during 21 days of training on the formation of endurance (running at middle distances), distributed on the main (15 people) and control groups (10 people). By design, the study was shaped like a blind placebo-controlled study and included the signing by the athletes of the “Informed Consent”. For comparison, the same indicators were studied in 10 donors of the same age and gender (men 19-22 years old). Results and discussion. Determination of the effect of ATP-long drug proved that its course application improved the prooxidant-antioxidant balance in erythrocyte membranes due to both inhibition of lipid peroxidation processes and an increase in the content of reduced glutathione in the membranes, the main non-enzymatic antioxidant, which led to an increase in the content of intraerythrocyte hemoglobin and indicated the release from the depot of young forms of red blood cells (an increase in the average volume of red cells and anisocytosis). In general, this indicates an improvement in the oxygen-transport function of the blood and indirectly indicates an increase in the degree of protection of athletes’ myocardium and skeletal muscle from ischemia and hypoxia. Conclusion. The results of the study proved the feasibility and validity of the course use of the metabolite ATP-long drug with aerobic exercise to maintain the red blood cell of hematologic homeostasis and improved the oxygen-transport function of the blood of athletes.

Keywords: aerobic load, red blood cell, membrane, energy supply, prooxidant-antioxidant balance

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  1. Akilov MV, Bobrovnik VI, Gunina LM. Guidelines on the problems of training Ukrainian athletes for the Games of the XXIX Olympic Games 2008 in Beijing. Nauka v Olimpiyskom Sporte. 2007; 3(Special issue): 1‒170. [Russian]
  2. Bankova BB, Prishchepova NF, Avratinsky OI. A method for assessing pathological changes in the plasma membrane in children with various diseases. Patologicheskaya Fiziologiya i Eksperimentvlnaya Terapiya. 1987; 3: 78–81. [Russian]
  3. Gunina LM Erythrocytes under oxidative stress at physical activity (a review). Perspektivi Meditsini ta Biologiyi. 2013; V(1): 7–13. [Ukrainian]
  4. Gunina LM, Oliynyk CA, Ivanov SV. Changes of blood indices and prooxidant-antioxidant balance in erythrocyte membranes under intense physical activity. Medichna Himiya. 2007; 9(1): 91‒9. [Ukrainian]
  5. Gunina LM, Vinnychuk YD, Nosach OV, Bezugla BB, Rosova KV. Mechanisms of the influence of ATP-long drug on the performance of animals in the experimental modeling of physical activity. Zhurnal Eksperimentalnih i Klinichnih Medichnih Doslidzhen. (JC&EMR). 2017; 5(3): 982–94. [Ukrainian]
  6. Gunina LM, Vinnichuk YuD, Sukhich VA, Gulay VS. Efficiency and safety of the use in sports of the metabolic cardioprotector ATP-long. Sovremennyie Zdorov’esberegayuschie Tehnologii. 2017; 2: 57–64. [Russian]
  7. Kuzmenko MV, Gunina LM The role of correction of energy generation processes in the prevention of functional disorders of the heart during exercise. Materiali vseukrayinskoyi naukovoyi konferentsiyi «Aktualni pitannya biologiyi ta meditsini». Sumy; 2017: 134‒6. [Ukrainian]
  8. Lang TA, Sesik M. Kak opisyivat statistiku v meditsine: rukovodstvo dlya avtorov, redaktorov i retsenzentov [How to describe statistics in medicine: a guide for authors, editors and reviewers]. M: Prakticheskaya meditsina; 2011. 480 s. [Russian]
  9. Lipkan GN, Mkhitryan LS, Kutnyak VN. ATP-Long is a representative of a new class of cardiotropic drugs. Zhurnal Praktikuyuchogo Likarya. 1999; 4: 56‒8. [Russian]
  10. Mazur I.A, Chekman IS, Belenichev IF, Gorchakova NA. Metabolitotropnyie preparatyi [Metabolitotropic drugs]. Zaporozhe; 2007. 309 s. [Russian]
  11. Osipov VP, Lukyanova EM, Antipkin SO. Metodika statisticheskoy obrabotki meditsinskoy informatsii v nauchnyih issledovaniyah [Methods of statistical processing of medical information in scientific research]. Ed by VP Osipov. Kiev: Planeta lyudey; 2002. 200 s. [Russian]
  12. Semko GA. Structural and functional changes in membranes and external membrane layers of erythrocytes during hyperepidermopoiesis. Ukrayinskiy Biohimichniy Zhurnal. 1998; 70: 113‒8. [Russian]
  13. Shvets NI, Davydov VV. Age-related features of changes in the glutathione system in the heart of rats with immobilization stress Ukrayinskiy Biohimichniy Zhurnal. 2008; 80(6): 74–8.
  14. Storozhshok SA, Sannikov AG, Zakharov YuM. Molekulyarnaya struktura membran eritrotsitov i ih mehanicheskie svoystva [The molecular structure of erythrocyte membranes and their mechanical properties]. Tyumen: Izdatelstvo TGU; 1997. 125 s. [Russian]
  15. Vsemirnyiy antidopingovyiy kodeks [World Anti-Doping Code]. M: RUSADA; 2015. 15 s. [Russian]
  16. Zaitsev VG, Zakrevsky VI, Davydov AI. Level of hyperglycemia in patients with diabetes mellitus. Klinicheskaya Laboratornaya Diagnostika. 1999; 11: 323. [Russian]
  17. Becatti M, Marcucci R, Mannucci A, Gori AM, Giusti B, Sofi F, et al. Erythrocyte Membrane Fluidity Alterations in Sudden Sensorineural Hearing Loss Patients: The Role of Oxidative Stress. Thromb Haemost. 2017; 117(12): 2334‒45.
  18. Connor B, Osborne W, Peir G, Smith M, John A. Factors Associated With Increased Exercise in Adults With Congenital Heart Disease. Am J Cardiol. 2019; 124(6): 947‒51.
  19. Cooper CE, Silkstone GGA, Simons M, Rajagopal B, Syrett N, Shaik T, et al. Engineering tyrosine residues into hemoglobin enhances heme reduction, decreases oxidative stress and increases vascular retention of a hemoglobin based blood substitute. Free Radic Biol Med. 2019; 134: 106‒18. 10.1016/j.freeradbiomed.2018.12.030
  20. Fernandes KS, Silva AH, Mendanha SA, Rezende KR, Alonso A. Antioxidant effect of 4-nerolidylcatechol and α-tocopherol in erythrocyte ghost membranes and phospholipid bilayers. Braz J Med Biol Res. 2013; 46(9): 780–8.
  21. Gunina Larisa, Vinnichuk Yulia, Rosova Ekaterina. Estimation of adenosine triphosphate based preparation influence on work capacity during modeling intensive continuous physical loads. Sporto Moklas. 2017; 3 (89): 27‒33.
  22. Huang NJ, Lin YC, Lin CY, Pishesha N, Lewis CA, Freinkman E, et al. Enhanced phosphocholine metabolism is essential for terminal erythropoiesis. Blood. 2018; 131(26): 2955-66.
  23. Kaesler A, Rosen M, Schmitz-Rode T, Steinseifer U, Arens J. Computational Modeling of Oxygen Transfer in Artificial Lungs. Artif Organs. 2018; 42(8): 786‒99.
  24. Martorell M, Pons V, Domingo JC, Capó X, Sureda A, Drobnic F, et al. Erythrocytes and Skeletal Muscle Unsaturated and Omega-6 Fatty Acids Are Positively Correlated after Caloric Restriction and Exercise. Ann Nutr Metab. 2018; 72(2): 126‒33.
  25. Quarmyne MO, Risinger M, Linkugel A, Frazier A, Joiner C. Volume regulation and KCl cotransport in reticulocyte populations of sickle and normal red blood cells. Blood Cells Mol Dis. 2011; 47(2): 95‒9.
  26. Schmied C, Borjesson M. Sudden cardiac death in athletes. J Intern Med. 2014; 275(2): 93‒103.
  27. Škrgat S, Korošec P, Kern I, Šilar M, Šelb J, Fležar M, Marčun R.. Systemic and airway oxidative stress in competitive swimmers. Respiratory Medicine. 2018; 137: 129‒33.
  28. Suzuki Y, Nakajima T, Shiga T, Maeda N. Influence of 2,3-diphosphoglycerate on the deformability of human erythrocytes. Biochim Biophys Acta. 1992; 1029(1): 85‒90.
  29. Tang J, Erdener SE, Fu B, Boas DA. Capillary red blood cell velocimetry by phase-resolved optical coherence tomography. Optics Letters. 2017; 42(19): 3976‒9.
  30. WADA Prohibited List-2019. International standard.