ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 47 of 60
УЖМБС 2019, 4(6): 316–320

Impact of the Humic Origin Supplement on the Antioxidant System of Rats Affected by Chromium (VI)

Buchko O. M. 1, Havryliak V. V. 2, Pylypets A. Z. 1

High ecological safety of humic substances, which are the basis of peat extracts, unique ability to improve metabolic processes in the body, to increase the energy of cells and to exhibit antioxidant properties, is used by scientists for the development of the preparation with a wide spectrum of action. The purpose of the study was to determine the effect of the biologically active feed supplement "Humilid" on the antioxidant defense system: activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, reduced glutathione content, and free radical processes: lipid hydroperoxidation products, TBK–active products, and carbonyl groups of protein in the blood of rats affected by Chromium (VI). Material and methods.White Wistar male rats with body weight 170–190 g were used in the experiment. Animals were divided into control and 2 experimental groups. The animals of D2 experimental group received 1% Humilide solution in an amount of 2 ml/kg of body weight during 28 days. From the 14th day of the experiment rats of D1 and D2 groups were intraperitoneally injected with potassium dichromate (K2Cr2O7) at a dose of 2 mg Cr (VI)/kg of body weight daily for 14 days. Animals of K group were intraperitoneally administered by 0.9% NaCl solution during14 days. Results and discussion. The obtained results showed that potassium (s) dichromate caused in the blood of rats oxidative stress, which was accompanied by a decrease in the activities of antioxidant enzymes superoxide dismutase and glutathione peroxidase, and a sharp increase in the content of lipid hydroperoxidation products, TBK–active products and carbonyl groups of the protein. The administration of Cr (VI) on the background of humic feed supplement led to a decrease in the concentration of metabolites of oxidative processes in the blood plasma of rats and the activation of antioxidant defense system in erythrocytes (increased levels of glutathione content and activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase). Conclusions. Humilid reduced the negative effect of potassium dichromate acting as an anti–stress supplement and activated the adaptation processes in the body of rats by inhibiting free–radical processes and stimulating antioxidant defense system. Inhibition of oxidative stress by the substances of humic origin justified their use in the prevention of metabolic disorders occuring after exposure of heavy metals.

Keywords: Humilid, Chromium, rats, antioxidant system, free–radical processes

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