This study helps exclude or confirm the terms and conditions causing abdominal trauma, especially under mysterious circumstances and events connected with repeated traumatic factor in the area of the small intestine. Material and methods. We studied forensic research acts, expert conclusions, and examined 120 fragments of sectional material consisting of hollow tissue with abdomen trauma. These were fragments of 87men and 33women aged from 20 to 60 in the presence and absence of alcohol in blood. To obtain the results we used histological and histochemical methods, performed statistical analysis of the results. Results and discussion. The obtained data showed the submucosal layer between muscle fibers and small hemorrhages in the form of clusters of red blood cells with indistinct contours, spasmodic artery with walls of blood vessels full-blooded. These results were found in fragments of the thoraco-abdominal trauma with damage to the colon, histological examination during an hour after caused injury. The adjacent mesentery of the colon presented stratum loose fibrous tissue that infiltrated erythrocytes mixed with rare leukocytes, and spasmodic vessel. Within 2-3 hours the mucous membrane of the swollen fibers were partially desquamated. In foci of hemorrhage submucosal layer of red blood cells there was a large number of white blood cells. In leykostazis vessels, leykodiapedezis and small clusters of white blood cells around the vessels. On the periphery of the cell layers there were bleeding, swollen, plethoric vessels. There were leukocytes, lymphocytes, macrophages, mast cells among small clusters of fibers. On the surface layers of the serous membrane there were alien particles of black color. Within 4-5 hours after injury there was a greater number of epithelial cells, with fuzzy contours nuclei, mucosa swollen, infiltrated leukocytes. In the layer we detected red blood cells hemorrhage with fuzzy contours, raised mixed leukocytes, on the periphery of hemorrhage in the area of reactive changes of muscle fibers there was a large number of white blood cells, lymphocytes, plasma cells, mast cells with cytoplasm. Serous membrane was swollen and moderately infiltrated leukocytes, with layers of fibrin strands mixed with a small amount of white blood cells. There were extensive vascular inflammatory infiltrates around the trauma. The mucosa of almost all epithelial cells got indistinct contours in the next 6-7 hours. There were fuzzy contours, mixed grain brown pigment in the submucosal layer in the cells of most of the hemorrhage of red blood cells, particularly in the center of hemorrhage. On the periphery of many pigmented macrophages plazmotsythis raised white blood cells, mast cells, which decrease in their number. Serosa layers of fibrin strands were sometimes with signs of organization, leukocytes, macrophages, including age macrophages. Conclusions. Thus, we found a regular dynamics of histological changes in the colon indicators of people who died due to injuries caused. This fact indicates the possibility of developing a set of criteria for assessing the occurrence of damage limitation setting gastrointestinal digestive tract, including the colon. In determining the time of injury we must take into account internal and external factors which could occur in the injured and the dead in different kinds of injuries, namely the presence of diseases, alcohol syndrome mutual charges, traumatic disease, individual characteristics of the organism, the circumstances and the mechanism of injury, concomitant damage and location, nature and extent of the damage of the colon.
Keywords: damage, abdominal, colon, mechanical injury, histological parameters
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