Perfecting the known and elaboration of new methods of periodontitis treatment is one of the important tasks and requires extraordinary approaches to their solutions. The purpose of the study was to determine effectiveness of water-soluble liposomal form of quercetin in correction of the glutathione system disorders in the experimental periodontitis development. The article presents the results of glutathione system researches in antioxidant defense, in particullarity, glutathione peroxidase, glutathione reductase activity and content of reduced glutathione in the rat’s blood serum on 14th days of the experimental periodontitis development, and after correction with flavonol quercetin, that was injected in doze 100 mg / kg for 7 days (from the 7th to the 14th day). The results were statistically processed using parametric and nonparametric statistical methods. The reliability of the differences in values between independent quantitative values was determined with a normal distribution according to the Mann-Whitney U criterion. The article shows a decrease enzymic activity of the glutathione system (glutathione peroxidase and reductase) observed at early stage of the inflammatory process with following preservation of their activity at reduced level. At the same time, content of reduced glutathione was decreased. The introduction of quercetin resulted in the increase of enzymes in the serum, and reduced glutathione and enzymes activity in the blood serum of experimental animal with periodontitis as compared to the indices of animals that were not administered this medicine. In addition, quercetin also contributed to the preservation of a pool of reduced glutathione in the animal blood. The development of the inflammatory process of the periodontal complex due to the combined effect of bacterial and immune factors is accompanied by dynamic changes in the activity of the glutathione system. Quercetin effectively stabilizes the prooxidant-antioxidant system during the course of experimental periodontitis, which is manifested by the increased activity of glutathione peroxidase and glutathione reductase and the increase in the content of reduced glutathione.

Keywords: Periodontitis, antioxidant defense, glutathione reductase, glutathione peroxidase, reduced glutathione, quercetin

Full text: PDF (Ua) 173K

1. Demkovych AYe, Bondarenko YuI. Patohenetychni osnovy modelyuvannya parodontytu u tvaryn. Zdobutky klinichnoi i eksperymentalnoi medytsyny. 2015; 1 (22): 54-7. [Ukrainian]
2. Kravets BB, Reheda MS. Rol korvitynu v korektsiyi porushen protsesiv peroksydnoho okysnennya lipidiv, aktyvnosti antyoksydantnoi systemy v krovi morskykh svynok pry eksperymentalnomu bakterialnomu keratyti na tli bronkhialnoi astmy ta pnevmoniyi. Medychna ta klinichna khimiya. 2015; 17(2): 69-71. [Ukrainian]
3. Bains VK, Bains R. The antioxidant master glutathione and periodontal health. Dent Res J. 2015; 12 (5): 389-405. https://www.ncbi.nlm.nih.gov/pmc/articles/4630702. https://www.ncbi.nlm.nih.gov/pubmed/26604952. https://doi.org/10.4103/1735-3327.166169
4. Berger RL, Casella C. Statistical Inference. 2nd ed. Florida: Duxbury Press; 2001. 374 p.
5. Chirumbolo S. The role of quercetin, flavonols and flavones in modulating inflammatory cell function. Inflamm. Allergy Drug Targets. 2010; 9: 263-85. https://www.ncbi.nlm.nih.gov/pubmed/20887269. https://doi.org/10.2174/187152810793358741
6. D'Andrea G. Quercetin: A flavonol with multifaceted therapeutic applications? Fitoterapia. 2015; 106: 256-71. https://www.ncbi.nlm.nih.gov/pubmed/26393898. https://doi.org/10.1016/j.fitote.2015.09.018
7. Demkovych AYe. The necrotic-apoptotic changes in blood mononuclear phagocytes in the experimental bacterial-immune periodontitis development. Svit medytsyny ta biolohiyi. 2018; 1 (63): 120-2.
8. Demkovych A, Bondarenko Yu, Hasiuk PA. Oxidative modification of proteins in the process of experimental periodontitis development. Interventional Medicine and Applied Science. 2017 Dec; 9 (4): 218-21. https://doi.org/10.1556/1646.9.2017.28
9. Dong YS, Wang JL, Feng DY, Qin HZ, Wen H, Yin ZM, Gao GD, Li C. Protective effect of quercetin against oxidative stress and brain edema in an experimental rat model of subarachnoid hemorrhage. Int J Med Sci. 2014; 11: 282-90. https://www.ncbi.nlm.nih.gov/pubmed/24516353. https://www.ncbi.nlm.nih.gov/pmc/articles/3917118. https://doi.org/10.7150/ijms.7634
10. Ďuračková Z. Some current insights into oxidative stress. Physiological Research. 2010; 59(4): 459-69. https://www.ncbi.nlm.nih.gov/pubmed/19929132
11. Kempuraj D, Madhappan B, Christodoulou S, Boucher W, Cao J, Papadopoulou N, Cetrulo CL, Theoharides TC. Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells. Br J Pharmacol. 2005; 145: 934-44. https://www.ncbi.nlm.nih.gov/pubmed/15912140. https://www.ncbi.nlm.nih.gov/pmc/articles/1576204. https://doi.org/10.1038/sj.bjp.0706246
12. Khorsavi Samani M, Poorsattar Bejeh A, Kashiri M, Gujeq D. Introducing cut-points for salivary nitric oxide to distinguish periodontitis from the normal periodontium. Minerva Stomatol. 2012; 61 (10): 443-8. https://www.ncbi.nlm.nih.gov/pubmed/23076026
13. Kruglikova GO, Stutman IM. Glutathione peroxidase and glutathione reductase activity of rat liver after the introduction of sodium selenite. Ukr Bioch J. 1976; 48(2): 227-33.
14. Kumar S, Pandey AK. Free radicals: health implications and their mitigation by herbals. British Journal of Medicine and Medical Research. 2015; 7 (6): 438-57. https://doi.org/10.9734/BJMMR/2015/16284
15. Lesjak M, Hoque R, Balesaria S, Skinner V, Debnam ES, Srai SK, Sharp PA. Quercetin Inhibits Intestinal Iron Absorption and Ferroportin Transporter Expression In Vivo and In Vitro. PLoS One. 2014; 9 (7): e102900. https://www.ncbi.nlm.nih.gov/pmc/articles/4109952. https://www.ncbi.nlm.nih.gov/pubmed/25058155. https://doi.org/10.1371/journal.pone.0102900
16. Li C, Zhang WJ, Choi J, Frei B. Quercetin affects glutathione levels and redox ratio in human aortic endothelial cells not through oxidation but formation and cellular export of quercetin-glutathione conjugates and upregulation of glutamate-cysteine ligase. Redox Biol. 2016; 9: 220-8. https://www.ncbi.nlm.nih.gov/pubmed/27572418. https://www.ncbi.nlm.nih.gov/pmc/articles/5011167. https://doi.org/10.1016/j.redox.2016.08.012
17. Machavariani A, Mazmishvili K, Grdzelidze T. Use of osteoplastic material to guide bone tissue regeneration defect. Georgian Med News. 2011; 201: 70-3.
18. Miles SL, McFarland M, Niles RM. Molecular and physiological actions of quercetin: need for clinical trials to assess its benefits in human disease. Nutrition Reviews. 2014; 72 (11): 720-34. https://www.ncbi.nlm.nih.gov/pubmed/25323953. https://doi.org/10.1111/nure.12152
19. Moffat JA, Armstrong PW, Marks GS. Investigations into the role of sulfhydryl groups in the mechanism of action of the nitrates. Canadian Journal of Physiology and Pharmacology. 1982; 60 (10): 1261-6. https://doi.org/10.1139/y82-185
20. Williams RJ, Spencer JP, Rice-Evans C. Flavonoids: antioxidants or signalling molecules? (review). Free Radical Biology & Medicine. 2004; 36 (7): 838-49. https://www.ncbi.nlm.nih.gov/pubmed/15019969. https://doi.org/10.1016/j.freeradbiomed.2004.01.001