ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 41 of 48
Up
УЖМБС 2018, 3(2): 237–244
https://doi.org/10.26693/jmbs03.02.237
Biology

Microbiota of the Bronchopulmonary Apparatus in the Exacerbation of the Obstructive Pulmonary Disease of Professional Etiology

Ivchuk V. V., Kovalchuk T. A.
Abstract

Microbial colonization or infection is one of the main causes of exacerbation of chronic obstructive pulmonary disease. The transition of obstructive pulmonary disease from the stage of remission to exacerbation is due to a gradual increase in the number of microorganisms in the respiratory tract. The purpose of the work was to establish the nature of the microbiological spectrum of sputum in the mining industry workers with chronic obstructive pulmonary disease of professional etiology. Materials and methods. Microbiological, microscopic, statistical. Results. It has been established that gram-positive (48.9 %), gram-negative (27.6 %) microorganisms and yeast-like fungi (23.5 %) were among the microbiota isolated during the course of chronic obstructive pulmonary disease of the professional etiology. Staphylococcus aureus strains (26.7 %), Candida albicans (22.3%), Klebsiella pneumoniae (10.9 %), Escherichia coli (9.3 %), Streptococcus viridans (5.7 %) and Streptococcus viridans (5.7 %) had a significant specific gravity Staphylococcus epidermidis (5.5 %). In the structure of the species composition of microorganisms isolated from sputum in patients with chronic obstructive pulmonary disease of the professional etiology, S. aureus (29.2 %), K. pneumoniae (12.5 %) and E. coli (9.7 % ) – for chronic obstructive pulmonary disease 1; S. aureus (31.8 %), E. coli (11.9 %) and K. pneumoniae (10.6 %) – for chronic obstructive pulmonary disease 2; S. aureus (18.8 %), K. pneumoniae (9.7 %) and S. epidermidis (9.7 %) – with chronic obstructive pulmonary disease 3. Conclusion. Character of bacterial and mycotic microflora that colonizes lower standard of patients on chronic obstructive pulmonary disease of professional etiology on the stage of intensifying is related to the clinical features and weight of disease flow. It is set that quantitative correlation, as among the gram-positive, such gram-negative microorganisms of distinguished for patients with chronic obstructive pulmonary disease of professional etiology, had considerable oscillation, depending on the stage of disease flow. The stamms of gram-positive cocci appear in patients’ sputum with chronic obstructive pulmonary disease of professional etiology on more early stages of disease from relatively by the favorable disease flow. Prospects for further research. An exposure and research of pathogens features at different stages of obstructive pulmonary disease flow of professional etiology for the workers of mining industry can extend an idea about motion of inflammatory process and help in realization of adequate therapy of this disease exacerbation.

Keywords: a chronic obstructive pulmonary disease, microbiocenosis of airways, mining industry

Full text: PDF (Ukr) 270K

References
  1. Basanets’ AV, Lubyanova IP. Profzakhvoryuvanist’ – aktual’na problema suchasnosti. Okhorona pratsi. 2011; 2: 42-5. [Ukrainian]
  2. Bysenova NM, Erhalyeva AS, Mytus NM. Etyolohycheskaya struktura mokroty bol’nykh s obostrenyem KHOBL. Klynycheskaya medytsyna Kazakhstana. 2013; 2: 13-8. [Russian]
  3. Bobrov SV, Shpahyna LA, Kuznetsova HV. Effektyvnost’ ranney dyahnostyky y profylaktyky khronycheskoy obstruktyvnoy bolezny lehkykh u rabotnykov promyshlennykh predpryyatyy (rezul’taty prospektyvnoho nablyudenyya). Medytsyna truda y promyshlennaya ekolohyya. 2011; 10: 6-10. [Russian]
  4. Zubkov MN. Moraxella catarrhalis: rol’ v patolohiyi lyudyny, identyfikatsiya ta antybiotykorezystentnist’. Infektsiyi ta antymikrobna khimioterapiya. 2001; 3: 38-41. [Ukrainian]
  5. Karpova ON, Punyn AA. Spektr etieolohichnykh znachushchykh mikroorhanizmiv u m’yazakh u khvorykh z zahostrennyam khronichnoyi obstruktyvnoyi khvoroby lehen’. Praktychna medytsyna. 2012; 1 (56): 81-4. [Ukrainian]
  6. Kundiyev YuI, Nahorna AM. Profesiyna zakhvoryuvanist’ v Ukrayini u dynamitsi dovhotryvaloho sposterezhennya. Ukrayins’kyy zhurnal z problem medytsyny pratsi. 2005; 1: 3-11. [Ukrainian]
  7. Mavrodyy VM. Antybakterial’na terapiya pry usunenni khronichnoho obstruktyvnoho zakhvoryuvannya lehen’. Ukrayins’kyy terapevtychnyy zhurnal. 2007; 1: 64-8. [Ukrainian]
  8. Nakaz MOZ Ukrayine N 555 vid 27.06.2013. «Pro zatverdzhennya ta vprovadzhennya medyko-tekhnolohichnykh dokumentiv zi standartyzatsiyi medychnoyi dopomohy pry khronichnomu obstruktyvnomu zakhvoryuvanni lehen’». Kyiv. 2013; 146. [Ukrainian]
  9. Prykaz N 535 Russia "Ob unyfykatsyy mykrobyolohycheskykh metodiv doslidzhennya, shcho zastosovuyut’sya v kliniko-diahnostychnykh laboratoriyakh likuval’no-profilaktychnykh zakladiv. Moskva. 1985; 126. [Russian]
  10. Rekalova EM. Vzayemozv’yazok mikroflory dykhal’nykh shlyakhiv z klinichnymy osoblyvostyamy khronichnoho obstruktyvnoho zakhvoryuvannya lehen’. Ukrayins’kyy pul’monolohichnyy zhurnal. 2005; 3: 23-6. [Ukrainian]
  11. Tets HV. Vybor antybyotykov pry smeshannykh ynfektsyyakh u patsyentov s khronycheskoy obstruktyvnoy bolezn’yu lehkykh. Praktycheskaya pul’monolohyya. 2015; 4: 21-5. [Russian]
  12. Alvarez-Sala JL, Kardos P, Martiner-Вeltran J, Coronel P, Aguilar L. Clinical and bacteriological efficacy in treatment of acute exacerbations of chronic bronchitis with cefditorenpivoxil versus cefuroximeaxetil. Antimicrob. Agents. Chemother. 2006; 50: 1762-7. https://www.ncbi.nlm.nih.gov/pubmed/16641447. https://www.ncbi.nlm.nih.gov/pmc/articles/1472220. https://doi.org/10.1128/AAC.50.5.1762-1767.2006
  13. Bari MR, Hiron MM, Zaman SM, Rahman MM, Ganguly KC. Microbes responsible for acute exacerbation of COPD. Mymensingh medical journal: MMJ. 2010; 19 (4): 576-85. https://www.ncbi.nlm.nih.gov/pubmed/20956903
  14. Beasley V, Joshi PV, Singanayagam A, Molyneaux PL, Johnston SL, Mallia P. Lung microbiology and exacerbations in COPD. International journal of chronic obstructive pulmonary disease. 2012; 7: 555-69. https://www.ncbi.nlm.nih.gov/pubmed/22969296. https://www.ncbi.nlm.nih.gov/pmc/articles/3437812. https://doi.org/10.2147/COPD.S28286
  15. Braeken DCW, Houben-Wilke S, Smid DE, Rohde GGU, Drijkoningen J JC, Wouters EFM, Spruit MA, Franssen FME. Sputum microbiology predicts health status in COPD. International journal of chronic obstructive pulmonary disease. 2016; 11: 2741-8. https://doi.org/10.2147/COPD.S117079
  16. Chambers DC, Gellatly SL, Hugenholtz Ph, Hansbro PhM. JTD special edition ´Hot Topics in COPD – The microbiome in COPD. Journal of thoracic disease. 2014; 6 (11): 1525-31. https://www.ncbi.nlm.nih.gov/pmc/articles/4255167. https://doi.org/10.3978/j.issn.2072-1439.2014.11.08
  17. Curran T, Coulter WA, Fairley DJ, McManus T, Kidney J, Larkin M, Moore JE, Coyle PV. Development of a novel DNA microarray to detect bacterial pathogens in patients with chronic obstructive pulmonary disease. Journal of Microbiology. 2010; 80 (3): 257-61. https://www.ncbi.nlm.nih.gov/pubmed/20074591. https://doi.org/10.1016/j.mimet.2010.01.004
  18. Dy R, Sethi S. The lung microbiome and exacerbations of COPD. Current opinion in pulmonary medicine. 2016; 22 (3): 196-202. https://www.ncbi.nlm.nih.gov/pubmed/26964078. https://doi.org/10.1097/MCP.0000000000000268
  19. Garcia-Nunez M. Bacterial and viral microbiomes in COPD exacerbations of unidentified cause. European Respiratory Journal. 2014; 44 (58): 2534.
  20. Garcia-Nunez M, Marti S, Puig C, Perez-Brocal V, Millares L, Santos S, Ardanuy C, Moya A, Liñares J, Monsó E. Bronchial microbiome, PA biofilm-forming capacity and exacerbation in severe COPD patients colonized by P. aeruginosa. Future Microbiology. 2017; 12 (5): 379-92. https://www.ncbi.nlm.nih.gov/pubmed/28339291. https://doi.org/10.2217/fmb-2016-0127
  21. Hassett DJ, Borchers MT, Panos RJ. Chronic obstructive pulmonary disease (COPD): evaluation from clinical, immunological and bacterial pathogenesis perspectives. Journal of Microbiology. 2014; 52 (3): 211-26. https://www.ncbi.nlm.nih.gov/pubmed/24585052. https://doi.org/10.1007/s12275-014-4068-2
  22. Langan CE, Craufield R, Briesch S, Pettit R. Randomized, double-blind stady of grepafloxacin versus amoxycillin in patients with acute bacterial exacerbations of chionic bronchitis. J. Antimicrob. Chemother. 1997; 40: 63-72. https://www.ncbi.nlm.nih.gov/pubmed/9484875. https://doi.org/10.1093/jac/40.suppl_1.63
  23. Leung JM, Tiew PY, Mac Aogáin M, Budden KF, Yong VF, Thomas SS4, Pethe K, Hansbro PM, Chotirmall SH. The role of acute and chronic respiratory colonization and infections in the pathogenesis of COPD. Respirology. 2017; 22 (4): 634-50. https://www.ncbi.nlm.nih.gov/pubmed/28342288. https://doi.org/10.1111/resp.13032
  24. Madhavi S. Bacterial etiology of acute exacerbations of chronic obstructive pulmonary disease. Journal of Microbiology and Biotechnology Research. 2017; 2 (3): 440-4
  25. Marin Monsó AE, Garcia-Nuñez M, Sauleda J, Noguera A, Pons J, Agustí A, Morera J. Variability and effects of bronchial colonisation in patients wich moderate COPD. European Respiratory Journal. 2010; 35 (2): 295-302. https://doi.org/10.1183/09031936.00126808
  26. Nseir S, Ader F, Lubret R, Marquette CH. Pathophysiology of airway colonization in critically ill COPD patient. Current drug targets. 2011; 12 (4): 514-20. https://www.ncbi.nlm.nih.gov/pubmed/21194404. https://doi.org/10.2174/138945011794751537
  27. Renom F, Yáñez A, Garau M, Rubí M, Centeno M-J, Gorriz M-T, Medinas M, Ramis F, Soriano JB, Agustí A. Prognosis of COPD patients requiring frequent hospitalization: role of airway infection. Respiratory medicine. 2010; 104 (6): 840-48. https://doi.org/10.1016/j.rmed.2009.12.010