ISSN 2415-3060 (print), ISSN 2522-4972 (online)
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УЖМБС 2017, 2(4): 189–193

Zinc Citrate Influence on Antioxidant Defence in Rats’ Liver and Pancreatic Gland during Experimentally Diabetes

Slivinska O. M.

The purpose of the article is to present data towards the zinc citrate action of on the activity of antioxidant system enzymes and the content of reduced glutathione and products of peroxide oxidation of lipids in liver and pancreatic tissues of rats under the condition of experimental streptozotocin – induced diabetes mellitus. The experiment was conducted on white laboratory rats, which were divided into four groups: I – the control group, II, III and IV – the experimental groups. Animals of the 3rd and 4th groups received zinc citrate solution in amount of 20 and 50 mg Zn/kg bodyweight dosage to a basic diet during a month. Experimental diabetes mellitus was induced in animals of all experimental groups after 24-hour fasting. Antioxidant defence indicators were determined in the homogenates of the liver and pancreas of the rats. There was a decrease in the activity of enzymes AOC and the content of reduced glutathione in tissues of animals of the group II compared to the group I – control, but in animals of groups III and IV the growth of the studied indices compared to the control group was observed. Researching the content of lipid hydroperoxides and TBC-active products in tissues of rats, the growth of their content in animals of the experimental group II compared to the control group was noticed. At the same time, in animals of III and IV groups, the levels of these indices were normalized, indicating the activation of antioxidant processes under the action of zinc citrate. The obtained results indicate that zinc citrate added to the diet of rats causes a decrease in lipid peroxidation processes in the studied tissues due to the activation of the antioxidant system. Due to its antioxidant properties, it can be used as a component of biologically active additives to prevent diabetes.

Keywords: rats, antioxidant system, diabetes mellitus, zinc citrate

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  1. Patent 1084681 SSSR, MKI G 01N 33/48. Sposob opredeleniya gidroperekisey lipidov v biologicheskikh tkanyakh / Mironchik VV (SSSR). № 3468369/2813; zayavl. 08.07.82; opubl. 07.04.84, byul. № 13. [Russian].
  2. Ataullakhanov FI, Zhabotinskiy AM, Pichugin AB. Zavisimost skorosti funktsionirovaniya pentoznogo puti v eritrotsitakh ot stepeni vosstanovlennogo glutationa. Biokhimiya. 1981; 46 (3): 530-40. [Russian].
  3. Vlizlo VV, Fedoruk RS, Makar IA, ta in. Dovidnik: Fiziologo-biokhimichni metodi doslidzhen u biologiyi, tvarinnitstvi ta veterinarniy meditsini. Lviv: Vidavnitstvo «VMS», 2004. 399 s. [Ukrainian].
  4. Dubinina EE, Salnikova LA, Efimova LF. Aktivnost i izofermentnyy spektr superoksiddismutazy eritrotsitov i plazmy krovi cheloveka. Laboratornoe delo. 1983; 10: 30-3. [Russian].
  5. Korobeynikova EN. Modifikatsiya opredeleniya POL v reaktsii s TBK. Laboratornoe delo. 1989; 7: 8-10. [Russian].
  6. Korolyuk MA, Ivanova LI, Mayorova IG, Tokarev VE. Metod opredeleniya aktivnosti katalazy. Laboratornoe delo. 1988; 1: 16-8. [Russian].
  7. Moin VM. Prostoy i spetsificheskiy metod opredeleniya aktivnosti glutationperoksidazy v eritrotsitakh. Laboratornoe delo. 1986; 12: 724-7. [Russian].
  8. Stefanov AV, Derimedved LV, Churilova IV, i dr. Klinikoeksperimentalnoe obosnovanie primeneniya preparatov superoksiddismutazy v meditsine. Kharkov: Izd-vo NFaU «Zolotye stranitsy», 2004. 288 s. [Russian].
  9. Bun SD, Guo YM, Guo FC, Ji FJ, Cao H. Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella Poultry Science. 2011; 90 (6): 1220-6.
  10. Eizirik DL, Darville MI. Beta-cell apoptosis and defence mechanisms : lesions from type 1 diabetes. Diabetes. 2001; 50 (Suppl 1): 64–9.
  11. Greenblatt HM, Feinberg H, Tucker PA, Shoham G. Carboxipeptidase A: native, zinc-removed and mercury- replaced forms. Acta Crystallogr D Biol Crystallogr. 1998; 54 (Pt 3): 289–305.
  12. Recent Advances and Clinical Application. Abstracts. International conference on Superoxide Dismutase. Institut Pasteur. Paris, May 1-15, 1998. 125 р.
  13. Kambe T, Yamaguchi-Iwai Y, Sasaki R, Nagao M. Overview of mammalian zinc transporters. Cell Mol Life Sci. 2004; 61: 49–68.
  14. Krebs NF. Overview of zinc absorption and excretion in the human gastrointestinal tract. J Nutr. 2000; 130: 1374–7.
  15. Kulikowska-Karpińska E, Moniuszko-Jakoniuk J. Lead and Zinc Influence on Antioxidant Enzyme Activity and Malondialdehyde Concentrations. Polish Journal of Environmental Studies. 2001; 10 (3): 161-5.
  16. Marcellini F, Giuli C, Papa R, et al. Zinc status, psychological and nutritional assessment in old people recruited in five European countries: Zincage study. Biogerontology. 2006; 7 (5-6): 339–45.
  17. McClain CJ, The pancreas and zinc homeostasis. J Lab Clin Med. 1990; 116: 275–6.
  18. Powell SR. The Antioxidant properties of zinc. J Nutr. 2000; 130: 1447S–54S.
  19. Raza H, Prabu SK, John A, Avadhani NG. Impaired mitochondrial respiratory functions and oxidative stress in streptozotocin-induced diabetic rats. International Journal of Molecular Sciences. 2011; 12 (5): 3133–47.
  20. Tate DJ, Miceli MV, Newsome DA. Zinc protects against oxidative damage in cultured human retinal pigment epithelial cells. Free Radic. Biol Med. 1999; 26: 704–13.