ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 10 of 58
JMBS 2020, 5(6): 79–83
Experimental Medicine and Morphology

Influence of Non-steroidal Anti-Inflammatory Drugs and Paracetamol on the Level of C-Reactive Protein of Rat Blood Serum in Experimental Equivalents of Hypothyroidism and Osteoarthritis

Nosivets D. S.

The article investigated changes in the level of C-reactive protein under the influence of non-steroidal anti-inflammatory drugs and paracetamol under experimental equivalents of hypothyroidism and osteoarthritis. There is a clear need to identify biomarkers that could predict a patient's response to osteoarthritis treatment, primarily in comorbid conditions. It is known that hypofunction of the thyroid gland leads to metabolic disorders that negatively affect the condition of bone and cartilage, causing the development of osteoarthritis. One manifestation of osteoarthritis is considered to be a pathological change in the subchondral bone, which responds to the disease by the formation of sclerosis, marginal bone growths and the formation of deformation of the joint surfaces due to the destruction of bone tissue. Although non-steroidal anti-inflammatory drugs are effective in reducing pain and disability in patients with osteoarthritis, it is still unclear to what extent these drugs can affect joint metabolism and, therefore, joint structure, especially against the background of functional thyroid insufficiency. The purpose of the study was to research the pharmacological activity of non-steroidal anti-inflammatory drugs and paracetamol on the level of C-reactive protein in the serum of rats with experimental equivalents of hypothyroidism and osteoarthritis. Material and methods. The experiments were carried out on 70 white outbred rats of both sexes, which recreated osteoarthritis and hypothyroidism. Experimental osteoarthritis was performed by single intra-articular administration of 0.1 ml of monoacetic acid solution in the knee joint, which was prepared at a rate of 3 mg of the reagent on 50 μl of sterile physiological saline. Experimental hypothyroidism was reconstructed by enteral administration of a 0.02% solution of carbimazole, which was prepared at a rate of 5 mg per 250 ml of physiological solution and given with a drinking ration of animals for 6 weeks. The adequacy of the model was confirmed by the level of serum TSH, T3 and T4 in rats. Results and discussion. After the formation of experimental models on the 42nd day of the experiment, the animals were divided into 14 groups and drug administration began daily for 5 days. The quantitative level of C-reactive protein of blood serum was determined by competitive in vitro ELISA twice on 42 and 47 days of the experiment. Blood samples were obtained from the rat tail vein by puncture using a vacuum system at 42 and 47 days of the experiment. Statistical data processing was performed using the Statistica 6.1 software package (StatSoftInc., Serial number AGAR909E415822FA) and included calculations of arithmetic mean values (M) and their errors (± m). The probability of the difference between the arithmetic mean (p) values of the indices was made using non-parametric U-criterion Mann-Whitney. The determination of the probability of intragroup and intergroup differences was performed using the parametric t-criterion of the Student and the method of single-factor dispersion analysis (ANOVA). Differences were considered statistically significant at p≤0.05. Conclusion. The author found that determining the level of C-reactive protein allowed evaluating the anti-inflammatory activity non-steroidal anti-inflammatory drugs on the background of experimental equivalents of osteoarthritis and hypothyroidism. The data obtained from rat’s serum C-reactive protein reflects the extent of the effects of non-steroidal anti-inflammatory drugs and paracetamol due to the interaction of drugs in experimental osteoarthritis and hypothyroidism. According to the degree of influence on degenerative-dystrophic processes in bone tissue the investigated drugs can be arranged as follows: diclofenac sodium > ibuprofen > nimesulide = meloxicam > celecoxib > paracetamol

Keywords: osteoarthritis, hypothyroidism, non-steroidal anti-inflammatory drugs, pharmacotherapy, biochemical markers, C-reactive protein

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  1. Nosyvets DS. Vlyianye funktsyonalnoi nedostatochnosty shchytovydnoi zhelezi na kostno-khriashchevuiu tkan [Influence of functional insufficiency of the thyroid gland on bone and cartilage tissue]. Morfolohyia. 2019; 1(13): 47-51. [Russian]
  2. Williams GR, Bassett JHD. Thyroid diseases and bone health. J Endocrinological Investigation. 2018; 41: 99-109.
  3. Ehmouda F, Eljazwi E, Eldrasi N. Effect of L thyroxine therapy on musculoskeletal symptoms of hypothyroidism. Lecture in faculty medicine in Benghazi University, Accepted In Pan Arab Rheumatology. 2014. p. 1-16.
  4. Graham RW. Thyroid hormone actions in cartilage and bone. Eur Thyroid J. 2013; 2: 3-13.
  5. Khimion LV, Klymas IV. Polimorbidnist pry revmatychnykh zakhvoriuvanniakh. Ukr revmatol zhurn. 2015; 4(62): 15-8. [Ukrainian]
  6. Oparin OA, Synelnyk VP. Problema komorbidnosti v klinichnii praktytsi likaria [The problem of comorbidity in the clinical practice of a doctor]. Visnyk VDNZU «Ukrainska medychna stomatolohichna akademiia». 2015; 4(52): 325-30. [Ukrainian]
  7. Voloshyna LO, Smiian SI. Osteoartroz, poli- ta komorbidnist: vikovi, henderni, prohnostychni y likuvalno-profilaktychni aspekty: dani tryrichnoho prospektyvnoho doslidzhennia [Osteoarthritis, poly- and comorbidity: age, gender, prognostic and treatment-and-prophylactic aspects: data from a three-year prospective study]. Ukr revmatol zhurn. 2016; 4(66): 51-7. [Ukrainian]
  8. Raval S, Joshi DV, Patel BJ, Desai GD. A dose response study of monosodium iodoacetate induced femorotibial osteoarthritis in Wistar rats. Indian J Vet Pathol. 2016; 40(4): 327-330.
  9. Nosivets DS. Eksperimentalnyie modeli patologii hryaschevoy tkani [Experimental models of cartilage tissue pathology]. Zaporozhskiy meditsinskiy zhurnal. 2019; 4(115): 554-60. [Russian]
  10. Ghanbari M, Ghasemi A. Maternal hypothyroidism: An overview of current experimental models. Life Sci. 2017; 15(187): 1-8.
  11. AVMA Guidelines for the Euthanasia of Animals: 2020 Edition. Available from:
  12. Rukovodstvo po provedeniyu doklinicheskih issledovaniy lekarstvennyih sredstv [Preclinical Drug Research Guide]. Pod red Mironova AN. Ch 1. M; 2012. 944 p. [Russian]
  13. Kostyuk VO. Prikladna statistika [Applied statistics]. Navch posibnik. H: HNUMG im OM Beketova; 2015. 191 p. [Ukrainian]
  14. Zupanets IA, Shebeko SK, Popov OS, Shalamay AS. Diclocor is superior to diclofenac sodiumand quercetin in normalizing biochemicalparameters in rats with collagen-inducedosteoarthritis. Inflammopharmacology. 2016; 24(1): 53-7.
  15. Won RSY. Disease-modifying effects of long-term and continuous use of nonsteroidal anti-inflammatory drugs (NSAIDs) in spondyloarthritis. Advances in Pharmacological Sciences. 2019; 1: 1-6. doi: 10.1155/2019/5324170