ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 44 of 56
JMBS 2018, 3(6): 279–287

State of the Antioxidant Protection System in Rats Erythrocytes under the Influence of Histamine and Sodium Hypochlorite

Harasym N. P., Bishko-Moskalyuk O. I., Wozniak M. V., Mandzynets S. M., Sanahursky D. I.

Sodium hypochlorite is used in medicine as a detoxifier due to oxidation of toxins and metabolites. This substance is officially used for disinfection of tap water. The widespread use of drugs for the release and histamine metabolism in medical practice is based on the use of two groups of drugs: histamine receptor blockers and plasma cell membrane stabilizing. However, the negative side effect causes the search for other safe paths of inactivation and reduction of histamine content in biological tissues. Thus, it is important to study the safety of the use of sodium hypochlorite for the treatment of patients with allergic manifestations. The excessive allocation of histamine, which is easily oxidized, mast cells and blood basophils should be also taken into account. Study of safety using the sodium hypochlorite for treatment the patients with allergic manifestation and the excessive allocation of histamine is an acute problematic nowadays. The purpose of the study was to investigate the influence of histamine (in doses of 1 and 8 µg/kg of body weight of animals) and sodium hypochlorite (in concentrations 5 and 20 mg/l), as well as their simultaneous action on the enzyme and non-enzymatic levels of the antioxidant system of red blood cells in rats. Material and methods. The test substances were administered to animals for 14 days. On the 1st, 7th, 14th and 21st (rehabilitation) days animals were decapitated. We took blood red cells samples by centrifugation and conducted their hemolysis. In hemolysates, the activity of glutathione peroxidase was studied, catalase, glutathione-S-transferase, amount of reduced glutathione. The dispersion analysis was also conducted. Results and discussion. The histamine in erythrocytes leads to a decrease in the activity of investigated enzymes and the content of reduced glutathione during the experiment, except for the 7th day of the experiment. Sodium hypochlorite causes the initial growth of activity of glutathione peroxidase with the next decreasing the activity. Glutathione-S-transferase is the least sensitive to the action of this substance. Sodium hypochlorite on the background of action of histamine disrupts the work of enzymes. At the first day of the experiment, a histamine influence on the activity of glutathione peroxidase, catalase, and the content of reduced glutathione was dominant, whereas the simultaneous administration of histamine and sodium hypochlorite was significantly influenced by glutathione-S-transferase activity. Conclusion. The combined administration of substances had a significant effect on the activity of glutathione peroxidase on the 7th and 14th days of the experiment. Sodium hypochlorite affected the activity of catalase and the content of reduced glutathione.

Keywords: histamine, sodium hypochlorite, erythrocytes

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  1. Buko IV, Polonetsky LZ, Mrochek AG, Moiseenok AG. Antioxidant status and glutathione redox potential of erythrocytes in patients with acute coronary syndrome. Ukr Biochem J. 2014; 86(3): 114-24. [Ukrainian]
  2. Gayda LM, Drobinska OV, Tymoshenko MA, Ostapchenko LI. The glutathione-dependent enzyme activity in rat parietal cells and hepatocytes under experimental atrophic gastrirtis. Physics of the Alive. 2008; 16(1): 144-8. [Ukrainian]
  3. Novik GA. Mechanisms of allergic reactions and methods of allergic examination in clinical practice. The educational-methodical manual is edited by the honored worker of science of the Russian Federation, professor IM Vorontsov. SPb: Publication GPMA; 2004. 76 p. [Russian]
  4. Wagner MC, Eckman JR, Wick TM. Histamine increases sickle erythrocyte adherence to endothelium. Br J Haematol. 2006; 132(4): 512-22.
  5. Ribas D, Fernández-Carranco MC, Hajji N, Bobadilla P, Monteseirín J. Eosinophil cationic protein and histamine production by neutrophils from patients with periodontitis. J Periodontol. 2018; 89(2): 228-34.
  6. Lebedev AT, Shaydullina GM, Sinikova NA, Harchevnikova NV. GC–MS comparison of the behavior of chlorine and sodium hypochlorite towards organic compounds dissolved in water. Water Res. 2004; 38: 3713-18.
  7. Chekman ES. Clinical pharmacology of antihistaminic drugs. Med of railw Transp of Ukr. 2002; 2: 58-61. [Ukrainian]
  8. Petrov SE. Application of sodium hypochlorite in clinical toxicology. Dissertation for the degree of Doctor of Medical Sciences. Moscow: 2005. [Russian]
  9. Bishko ОI, Harasym NP, Sanahursky DI. The system of antioxidant defence in liver and kidneys of rat at influence of histamine and sodium hypochlorite. Exper Clin Physiol Biochem. 2014; 3: 33-43. [Ukrainian]
  10. Bishko ОI, Harasym NP, Sanahurs’kyj DI. Antioxidant defense system state in blood plasma and heart muscle of rats under the influence of histamine and sodium hypoclorite. Uk Biochem J. 2014; 86(6): 56-65. [Ukrainian]
  11. Komarenko VI, Terekhov AA, Vorobyova AP. Investigation of the role of H1-receptor responses in rat liver portal vessels to histamine. Cherk Nat Univ B Khmelnitsky Biol Sci Series. 2008; 128: 54-8. [Ukrainian]
  12. Moin VM. A simple and specific method for determining the activity of glutathione peroxidase in erythrocytes. Lab Bus. 1986; 2: 724-7. [Russian]
  13. Koroljuk MA, Ivanovа LI, Mayorov IG. Method for determination of catalase activity. Labor Work. 1988; 1: 16-9. [Russian]
  14. Habig WH, Parst MJ, Jakobv WB. Glutathione-S-transferases. The first enzymatic step in mercapturie acid formation. Journal of Biological Chemistry. 1974; 249(22): 7130-9.
  15. Sibirna NO, Majevska OM, Barska ML. Investigation of certain biochemical indices under conditions of oxidative stress. Lviv: Publishing Center of І Franko LNU; 2006. 60 p. [Ukrainian]
  16. Apihtina OL. Investigation of membrane toxic effect of heavy metals on the model of erythrocytes of blood in vitro. Modern problems of toxicology. 2011; 1-2: 65-9. [Ukrainian]
  17. Borovskaya MK, Kuznetsov EE, Gorokhova VG, Koriakina LB, Kurilskaya TE, Pivovarov JuI. Structural and functional characteristics of membrane’s erythrocyte and its change at pathologies of various genesis. Bulletin VSSC of the RAMS. 2010; 3(73): 334-54. [Russian]
  18. Taysi S, Ucuncu H, Elmastas M, Aktan B, Emin Buyukokuroglu M. Effect of melatonin on lipid peroxidation, glutathione and glutathione-dependent enzyme activities in experimental otitis media with effusion in guinea pigs. J Pineal Res. 2005; 39(3): 283-6.
  19. Ucuncu H, Taysi S, Aktan B, Buyukokuroglu ME, Elmastas M. Effect of dantrolene on lipid peroxidation, lutathione and glutathione-dependent enzyme activities in experimental otitis media with effusion in guinea pigs. Hum Exp Toxicol. 2005; 24 (11): 567-71.
  20. Stupin FP, Tatkov OV. General blood analysis. Information collection. Yekaterinburg: Publishing solutions, 2016. 75 p. [Russian]
  21. Kang DH, Kang SW. Targeting Cellular Antioxidant Enzymes for Treating Atherosclerotic Vascular Disease. Biomolecules and Therapeutics. 2013; 21(2): 89-96.
  22. Espinoza SE, Guo H, Fedarko N, DeZern A, Fried LP, Xue QL, Leng S, Beamer B, Walston JD. Glutathione рeroxidase еnzyme аctivity in аging. J Gerontol A Biol Sci Med Sci. 2008; 63(5): 505-9.
  23. Fedets OM. Structure and functions of glutathione transferases. Ukr Biochem J. 2014; 86(3): 23-32. [Ukrainian]
  24. López-Galindo C, Vargas-Chacoff L, Nebot E, Casanueva JF, Rubio D, Solé M, Mancera JM. Biomarker responses in Solea senegalensis exposed to sodium hypochlorite used as antifouling. J Chemosphere. 2010; 78(7): 885-93.
  25. López-Galindo C, Vargas-Chacoff L, Nebot E, Casanueva JF, Rubio D, Mancera JM, Solé M. Sublethal responses of the common mussel (Mytilus galloprovincialis) exposed to sodium hypochlorite and Mexel432 used as antifoulants. Ecotoxicol Environ Saf. 2010; 73(5): 825-34.