The purpose of the study is to compare the effectiveness of probiotics to restore the microflora of women. Materials and methods. Correction of the microflora of the urogenital tract in dysbacteriosis in the development of various pathological conditions is one of the fundamental conditions for comprehensive treatment of the underlying disease. The urogenital microflora of 80 voluntary women of different ages was examined using the real-time polymerase chain reaction method, who applied to the Medical Diagnostic Center of the Medical Academy (Dnipro) with various dysbiotic disorders. For this purpose, samples from the vagina were taken from women aged 19 to 55 years. The real-time polymerase chain reaction method was used. With the help of this method it is possible to objectively and accurately assess the system of biocenosis of the vagina in a short time by quantitative and qualitative assessment of different groups of microorganisms and identify the relationship between them. Results and discussion. A study of the antagonistic activity of probiotic strains: L. plantarum («Lactobacterin dry»), L. acidophilus («Acilact»), L. rhatnosus 573 («Bioselak»), L. reuteri RC-14 («Vagilak») was carried out as for the condition-pathogenic clinical strains S. aureus, E. coli, P. vulgaris, G. vaginalis, C. albicans, detected in dysbiotic disorders of the urogenital tract of women using the method of perpendicular strokes. The probability of differences between the compared values was estimated using Student's t-test. To correct the microflora, probiotic preparations containing lactose-containing strains were used: L. plantarum («Lactobacterin dry»), L. acidophilus («Acilact»), L. rhatnosus 573 («Bioselak»), L. reuteri RC-14 («Vagilak»). The analysis of research results was performed using statistical data processing methods. Statistical processing of the results was performed using Microsoft Excel programs. According to the results of the study, it was found that among 80 patients, 30 (37.5%) had a normocenosis. In 50 of them (62.5%) various pathological conditions of the urogenital tract of women caused by opportunistic pathogens were identified. It has been proven that in dysbiotic disorders of the microflora of the urogenital tract there is a decrease in the amount of Lactobacillus spp. from to CFU/ml, against the background of increased S.aureus, E. coli, P. vulgaris, G. vaginallis, Megashera spp., Mobiluncus spp., Peptostreptococcus spp., Candida spp. in an amount from to CFU/ml. It was determined that the strains of Lactobacillus rhamnosus 573, Lactobaccillus reuteri RC-14, isolated from probiotic preparations against opportunistic pathogens Escherichia coli, Staphylococcus aureus, Proteus vulgaris, showed the greatest antagonistic activity. It was shown that cultures of Lactobacillus reuteri RC-14 and Lactobacillus rhamnosus 573 showed high activity against S. aureus (10.25 mm±0.2 mm), E. coli (9.75 mm ± 0.4 mm), P. vulgaris (9.8 mm ± 0.4 mm), G. vaginalis (9.05 mm ± 0.25 mm), C. albicans (9.75 mm ± 0.4 mm). Cultures of Lactobacillus plantarum and Lactobacillus acidophilus were less antagonistically active, with the exception of S. aureus and P. vulgaris, the zones of suppression of their growth were 8.7 mm ± 0.3 mm and 7.4 mm ± 0.3 mm. Conclusion. Positive dynamics of recovery of women urogenital tract microbiota at the use of probiotic drugs «Bioselak» and «Vagilak» is revealed. «Vagilak» probiotic proved to be more effective. The percentage of recovery of lactobacilli was 87%, with the use of «Bioselak» probiotic this figure was 69.6%

Keywords: vaginal microbiocenosis, dysbiosis, lactobacilli, antagonistic activity, probiotics

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