ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 25 of 33
Up
JMBS 2021, 6(4): 179–185
https://doi.org/10.26693/jmbs06.04.179
Biology

Experimental Estimation of Embryotoxic Risks of Cadmium Chloride with Isolated Administration and in Combination with Salts of Citrate and Nanocomposite on its Basis

Kolosova I. I. 1, Slesarenko E. G. 2, Myasoyed Yu. P. 2, Kovtunenko R. V. 2, Titov G. I. 2, Rukavishnikova D. K. 2, Evtushenko N. V. 2
Abstract

Cadmium compounds that enter living organisms pose the potential for serious health effects as they exhibit mutagenic, carcinogenic, gonadotoxic, teratogenic, allergenic and embryotoxic effects. It is important to study the mechanisms of their toxic effect on embryonic development and structural and functional formation of organs of living organisms and to search for their bioantogonists. The purpose of the study was to highlight the results of intragastric administration of cadmium chloride (1.0 mg/kg) with isolated administration and in combination with metal citrates (cerium, germanium, zinc, and nanocomposite (iodine + sulfur + selenium citrates)) on the overall course of rat embryogenesis. Materials and methods. For the study, young female Wistar rats weighing 170-200 g were used. They were divided into 6 groups, depending on the test substances, which they received from the 1st to the 19th day of gestation. Results and discussion. The analysis of the results of the experimental study showed the negative effect of cadmium chloride on the analyzed indicators of embryogenesis (preimplantation mortality, postimplantation mortality, total embryonic mortality), indicators of intrauterine survival, morphological (anatomical) malformations, as well as general delay in fetal development), both on the 13th and 20th days of pregnancy relative to the control group. In particular, the indicators of total embryonic mortality significantly increased by 4.2 times (13th day) and 3.7 times (20th day), with significant increase preimplantation mortality by 6.5 times, postimplantation mortality by 3.0 times on the 13th day of embryonic development, and on the 20th day, the preimplantation mortality indicator was by 14.0 times higher (p <0.01), and the postimplantation mortality indicator was insignificantly by 2.5 times higher than the control group. In the groups of the combined effect of cadmium chloride with metal citrates, there was a proven decrease in the indicators of total embryonic mortality (18.18% - 38.10%), preimplantation mortality (21.43% - 53.85%), postimplantation mortality (20.0% - 66.7%), an increase in the number of fetuses per female (12.66% -36.0%). Conclusion. In groups of the combined effect of cadmium chloride with metal citrates, the obtained data indicate a decrease in the accumulation of cadmium under the influence of the studied citrates, which allows them to be considered as potential bioantagonists of cadmium chloride

Keywords: embryogenesis, embryonic mortality, cadmium chloride, experiment, citrates

Full text: PDF (Ukr) 340K

References
  1. Human Biomonitoring: facts and figures. Kopenhagen: European Regional Office WHO. 2015. Available from: https://www.euro.who.int/__data/assets/pdf_file/0007/276388/Human-biomonitoring-facts-figures-ru.pdf [assessed: 30.05.2021]
  2. Skalny AV. Otsenka i korrektsiya elementnogo statusa naseleniya – perspektivnoe napravlenie otechestvennogo zdravookhraneniya i ekologicheskogo monitoringa [Assessment and correction of the elemental status of the population is a promising area of domestic health care and environmental monitoring]. Microelements in medicine. 2018; 19(1): 5-13. [Russian] https://doi.org/10.19112/2413-6174-2018-19-1-5-13
  3. Skalny AV, Zaitseva IP, Tinkov AA. Mikroelementy i sport. Personalizirovannaya korrektsiya elementnogo statusa sportsmenov [Microelements and sports. Personalized correction of the elemental status of athletes]. M: Sport; 2018. 288 p. [Russian]
  4. Gzhegotskiy MR, Sukhodol'skaya NV. Vliyaniye medi, tsinka, kadmiya i svintsa na veroyatnost' razvitiya ugrozy preryvaniya beremennosti u zhenshchin [The effect of copper, zinc, cadmium and lead on the likelihood of the threat of pregnancy interruption in women]. Reproduktivnoye zdorov'ye Vostochnoj Evropy. 2014; 1(31): 43–9. [Russian]
  5. United States Environmental Protection Agency. Chemicals and Toxics Topics [Internet]. USA: EPA; 2021 [updated 2021 May 29]. Available from: https://www.epa.gov/environmental-topics/chemicals-and-toxics-topics
  6. Gull, Dar AA, Sharma M. Effects of Heavy Metals on the Health of Pregnant Women and Fetus: A Review. Int J Theoret Applied Sci. 2018; 10(1): 1-9.
  7. Kosolapov VM, Chuykov VA, Khudyakova KHK, Kosolapova VG. Mineral'nyye elementy v kormakh i metody ikh analiza [Mineral elements in feed and methods of their analysis]. M: OOO «Ugreshskaya tipografiya»; 2019. 272 р. [Russian]
  8. Nefʹodov OO, Bilyshko DV, Kushnarova KA, Shevchenko OS, Shatorna VF, Kefeli-Yanovska OI, et al. Vyznachennya vplyvu kadmiyu na pokaznyky embriohenezu pry izolʹovanomu vvedenni ta v kombinatsiyi z tsytratamy selenu ta hermaniyu [Determination of the impact of cadmium on indicators of embryogenesis with isolated administration and in combination with selenium citrates and germanium]. Medicni perspektivi. 2020; 25(1): 24-31. [Ukrainian] https://doi.org/10.26641/2307-0404.2020.1.200395
  9. Rajakumar S, Abhishek A, Selvam GS, Nachiappan V. Effect of cadmium on essential metals and their impact on lipid metabolism in Saccharomyces cerevisiae. Cell Stress Chaperones. 2020; 25(1): 19-33. https://www.ncbi.nlm.nih.gov/pubmed/31823289. https://www.ncbi.nlm.nih.gov/pmc/articles/6985397. https://doi.org/10.1007/s12192-019-01058-z
  10. Li Y, Kimura T, Huyck RW, Laity JH, Andrews GK. Zinc-induced formation of a coactivator complex containing the zinc-sensing transcription factor MTF-1, p300/CBP, and Sp1. Mol Cell Biol. 2008; 28: 75–84. https://www.ncbi.nlm.nih.gov/pubmed/18458062. https://www.ncbi.nlm.nih.gov/pmc/articles/2447150. https://doi.org/10.1128/MCB.00369-08
  11. Marushko YuV, Asonov AO Klinichne znachennya defitsytu tsynku u ditey iz khronichnym hastroduodenitom, asotsiyovanym iz Helicobacter pylori [Clinical value of zinc deficiency in children with chronic gastroduodenitis associated with Helicobacter Pylori]. Dytyachyy likar. 2015; 3-4(40-41): 5-8. [Ukrainian]
  12. Lamas GA, Navas-Acien A, Mark DB, Lee KL. Heavy metals, cardiovascular disease, and the unexpected benefits of edetate chelation therapy. J Am Coll Cardiol. 2016; 67: 11-18. https://www.ncbi.nlm.nih.gov/pubmed/27199065. https://www.ncbi.nlm.nih.gov/pmc/articles/4876980. https://doi.org/10.1016/j.jacc.2016.02.066
  13. Yildiz A, Kaya Y, Tanriverdi O. Effect of the Interaction Between Selenium and Zinc on DNA Repair in Association with Cancer Prevention. J Cancer Prev. 2019; 24: 46–54. https://www.ncbi.nlm.nih.gov/pubmed/31624720. https://www.ncbi.nlm.nih.gov/pmc/articles/6786808. https://doi.org/10.15430/JCP.2019.24.3.146
  14. Chen F, Wu K, Yang Y, Xu M. Study on iodine nutrition and related influencing factors of children aged 8-10 in Wuhan. Chinese journal of school health. 2021; 42(1): 32-36. https://doi.org/10.16835/j.cnki.1000-9817.2021.01.008
  15. Kiseleva LG, Khar'kova OA, Chumakova GN. Soderzhaniye tyazhelykh metallov v mekonii novorozhdennykh u kuryashchikh materey [The content of heavy metals in the mefony of newborns in smoking mothers]. Ekologiya cheloveka. 2015; 7: 20-26 https://doi.org/10.33396/1728-0869-2015-7-20-26 [Russian]