ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 38 of 67
JMBS 2020, 5(3): 282–289

Anti-Adhesive Properties Metabolites Complexes of Lactobacillus rhamnosus GG and Saccharomyces boulardii in Tests in vitro

Isayenko O. Yu. 1, Knysh O. V. 1, Minukhin V. V. 1, Ryzhkova T. N. 2, Dyukareva G. I. 3

Along with the relevance of preventing the formation of biofilms, their damage, antimicrobial action on fungi and bacteria, especially antibiotic-resistant, it is important to find substances that can inhibit the adhesion process, since attachment of microbial cells of the pathogen to the biological object is the beginning of the development infectious process and primary stage of formation of biofilm of pathogens, which are much more complex to struggle than planktonic forms. The purpose of the work is to study in vitro the ability of metabolites complexes of Lactobacillus rhamnosus GG and Saccharomyces boulardii, obtained by the author's method, to influence the adhesion of microbial cells to substantiate the prospects of their use in the development of new multifunctional preparations. Material and methods. The in vitro study of the adhesion of bacterial cells of Staphylococcus aurues 209-P was carried out after their treatment or treatment of erythrocytes with disintegrates (obtained by ultrasonic irradiation of cells of Lactobacillus rhamnosus GG or Saccharomyces boulardii) and metabolites (obtained by culturing lactobacteria and saccharomycetes in their own structural components). Results and discussion. The highest statistically significant inhibition of adhesion of the treated S. aurues cells occurred with L. rhamnosus metabolites obtained by culturing producers in their own structural components (17.19%, P=0.0005) and S. boulardii metabolites obtained by culturing producents in structural components L.°rhamnosus (11.03%, P=0.006). The maximum suppression of adhesion of S. aurues during the processing of erythrocytes was also carried out by metabolites of L. rhamnosus (by 14.9%, P=0.005) and metabolites of S. boulardii (by 12.98%, P=0.02). Regardless of the treatment method (erythrocytes or staphylococcus cells) less anti-adhesive properties had the disintegrate of lactobacteria and the combination of metabolites L. °rhamnosus and S. boulardii (by 4.12% - 5.63%). The absence of suppression of the adhesion of pathogen cells to erythrocytes was noted during the treatment of staphylococcus with metabolites of S. boulardii, obtained by culturing the producents in their own structural components, and during the processing of erythrocytes also by the disintegrate of S. boulardii. Conclusion. The presented results can be useful when creating antimicrobial preparations new generation based on the metabolite complexes Lactobacillus and Saccharomyces.

Keywords: adhesion, metabolites, anti-adhesive properties, Lactobacillus rhamnosus GG, Saccharomyces boulardii

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