Experimental Medicine and Morphology
Histomorphometric Analysis of Different Phases of Skeletal Muscle Regeneration after Mechanical InjuryRtail R. A.
- Sumy State Univercity, 2, Rymskogo-Korsakova St., Sumy 40007, Ukraine
It is known that process of skeletal muscle recovery differs in each individual organism and depends on the damage degree and damaging factor nature. Along with this, one of the problems in describing skeletal myogenesis structural features is difficulty of clear determining morphometric parameters that can be used for statistical comparative analysis.
The purpose of research was to study the histomorphometric features of different stages of skeletal muscle regeneration in rats after mechanical injury.
Materials and methods. 50 laboratory white male rats (7–9 months) were used for the study. Control rats (10 animals) were not subjected to any manipulation. Triceps surae muscle mechanical injury was reproduced in experimental group animals (40 rats). Structural features of skeletal muscle recovery were studied on the 3rd, 7th, 14th and 28th days after mechanical injury. Light microscopy was performed using Olympus BH–2 microscope (Japan). Morphometric analysis included determination of different muscle fibers number, their cross–sectional area, connective tissue area, damage area and regeneration area. SPSS–17 software package was used for statistical analysis. The reliability of differences in morphometric parameters between four terms of experiment was performed using one–way ANOVA for repeated measurements. The difference was considered significant if the P value did not exceed 0.05 (P < 0.05).
Results and discussion. On the 28th day of experiment the defect site in experimental animals was filled with connective tissue and muscle fibers. Morphometric analysis showed that all muscle fibers relative area (P<0.001), connective tissue relative area (P<0.001), damage area (P<0.001), and regeneration area (P<0.001) differed significantly at different stages of the experiment both in pericentral and in marginal zones of regeneration site.
Conclusion. The obtained results showed that regenerative process of rats striated muscles after mechanical injury was completed by the formation of connective tissue–muscle regenerate. At the same time, in different parts of muscle defect, there was a decrease of connective tissue area and damage area, while regeneration area and muscle fibers total area were increased till the completion of skeletal muscles recovery process.
Keywords: muscle regeneration, mechanical trauma, morphometric analysis
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