ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 47 of 56
Up
JMBS 2018, 3(6): 299–303
https://doi.org/10.26693/jmbs03.06.299
Biology

The Fluorescent Probe Method in Investigation of the State of Erythrocyte Membranes in White Rats at Exposure to Chemical Environmental Factors

Nakonechna O. A.1, Bezrodna A. I.1,2, Kornienko E. M.2, Tkacheva T. N.3, Efimova S. L.3, Posokhov E. A.4, Maksimova I. G.1
Abstract

The purpose of the presented work was to study the possibility of using the fluorescent probes method to diagnose the harmful effects of chemical factors on the example of polyethylene glycol on the organism of white rats by evaluating the state of erythrocyte membranes. Material and methods. We used the following fluorescent probes in the studies: ortho-hydroxy derivatives of 2.5-diaryl-1.3-oxazole. Results and discussion. In the case of erythrocytes of rats, which are toxic to PEG-400, there is a marked decrease in the fluorescence intensity of all the probes used. The discussed decrease in the fluorescence intensity of the probes indicates a decrease in the number of molecules of each of the probes associated with erythrocyte membranes per one hour of incubation, which indicates a decrease in the rate of binding of probes to membranes. Such a decrease in the binding rate can be explained by the formation of an additional protective membrane around each lipid membrane. Conclusion. The established fact of formation of an additional coat of polyethylene glycol molecules on the surface of erythrocyte membranes can be treated as a stable standardized indicator for the method of fluorescent probes, which may indicate the absence of damaging effect on the membranes at the object of study, which requires confirmation in further studies.

Keywords: chemical factors, polyethylene glycol, toxification, erythrocytes, biomembrane, lipid bilayer, fluorescent probe

Full text: PDF (Eng) 280K

References
  1. Vinogradova SV, Vasnev VA Polycondensation processes and polymers. Moskva: Nauka; 2000. 373 p. [Russian]
  2. Eerkes-Medrano D, Thompson RC, Aldridge DC. Microplastics in freshwater systems: a review of the emerging threats, identification of knowledge gaps and prioritisation of research needs. Water Research. 2015; 75: 63-82. https://www.ncbi.nlm.nih.gov/pubmed/25746963. https://doi.org/10.1016/j.watres.2015.02.012
  3. Horn O, Nallia S, Coopera D. Plasticizer metabolites in the environment.  Water Research. 2004; 38(17): 3693-98. https://www.ncbi.nlm.nih.gov/pubmed/15350420. https://doi.org/10.1016/j.watres.2004.06.012
  4. Nakonechna O A, Marakushyn DI, Stecenko SA. Modern ideas about the mechanisms of adaptation to the action of xenobiotics. Eksperymentalna i klinichna medycyna. 2013; 4(61): 29-33. [Russian]
  5. Nakonechna OA, Komarevceva IO, Zhernovaya MYe. Effect of polyoxypropylene glycol molecular weight 500 (L-502-2-10) on the state of biological membranes under conditions of long subtoxic influence on the body of rats. Materials of the XI International Scientific and Practical Conference "Prospects of World Science". 2015: 60-7.
  6. Muncke J, Backhaus T,Geueke B, Maffini MV, Martin OV, Myers JP, et al. Scientific Challenges in the Risk Assessment of Food Contact Materials. Environ Health Perspect. 2017 Sep 11; 125(9): 095001. https://www.ncbi.nlm.nih.gov/pubmed/28893723. https://www.ncbi.nlm.nih.gov/pmc/articles/5915200. https://doi.org/10.1289/EHP644.
  7. Dyiment ON. Glycols and other derivatives of oxides of ethylene and propylene. Moskva: Himiya; 1976. 373 p. [Russian]
  8. Severin SE, Soloveva GA Workshop on Biochemistry. Moskva: Izdatelstvo MGU; 1989. 509 p. [Russian]
  9. Doroshenko AO, Posokhov EA. Proton phototransfer in a series of ortho-hydroxy derivatives of 2,5-diphenyl-1,3-оxazole and 2,5-diphenyl-1,3,4-оxadiazole in polystyrene films. Theor Exper Chem. 1999; 35: 334-7. [Russian]
  10. Doroshenko AO, Posokhov EA, Shershukov VM, Mitina VG, Ponomarev OA. Intramolecular proton-transfer reaction in an excited state in a series of ortho-hydroxy derivatives of 2,5-diaryloxazole. High Energy Chemistry. 1997; 31(6): 388–94. [Russian]
  11. Doroshenko AO, Posokhov EA, Verezubova AA, Ptyagina LM. Excited state intramolecular proton transfer reaction and luminescent properties of the ortho-hydroxy derivatives of 2,5-diphenyl-1,3,4-oxadiazole. J Phys Org Chem. 2000; 13: 253-65. [Russian]
  12. Doroshenko AO, Posokhov EA, Verezubova AA, Ptyagina LM, Skripkina VT, Shershukov VM. Radiationless deactivation of the excited phototautomer form and molecular structure of ESIPT-compounds. Photochem Photobiol Sci. 2002; 1: 92-9. [Russian]
  13. Posokhov EA, Abmanova NA, Boyko TP, Doroshenko AO. Ortho-hydroxy derivatives of 2,5-diphenyl-1,3-oxazole and 2,5-diphenyl-1,3,4-oxadiazole as fluorescent probes for medical and biological research. Kharkov University Bulletin. 1999; 454: 188-90. [Russian]
  14. Posokhov YO. Ortho-hydroxy derivatives of 2,5-diphenyl-1,3-оxazole and 2,5-diphenyl-1,3,4-оxadiazole as fluorescent probes for toxicological study of the cells of olfactory analyzer of rats. Kharkov University Bulletin. Chemical Series. 2011; 20(43): 92-9. [Russian]
  15. Posokhov YO. Ortho-hydroxy derivatives of 2,5-diphenyl-1,3-оxazole and 2,5-diphenyl-1,3,4-оxadiazole as fluorescent probes for toxicological investigations of model biomembranes. Kharkov University Bulletin. Chemical Series. 2001; 7(30): 192-94. [Russian]
  16. Posokhov YeO, Tkachenko AS, Korniyenko YeM. Influence of carrageenan (E 407) on the membrane of enterocytes investigated by fluorescent probes. Bulletin of Problems in Biology and Medicine. 2013; 1 (1/98): 229–33. [Russian]
  17. Dobretsov GE. Fluorescence probes in cell, membrane and lipoprotein investigations. Moscow: Nauka; 1989. 277 p. [Russian]
  18. Babiychuk LA, Zemlyanskih NG. Influence of polyethylene oxide-1500 and of temperatute on pecularities of modification of erythrocyte membranes. Problems of Criobiology. 1996; 4: 30-8. [Russian]
  19. Nardid OA, Cherkashina YaO, Ivanov LV, Nardid EO, Lyapunov AN, Mamontov VV. Effect of propylene glycol and polyethylene glycol with molecular weight of 1,500 on erythrocyte membrane microviscosity. Problems of Criobiology and Cryomedicine. 2016; 26(1): 35–44. [Russian]