ISSN 2415-3060 (print), ISSN 2522-4972 (online)
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УЖМБС 2018, 3(3): 53–59
https://doi.org/10.26693/jmbs03.03.053
Experimental Medicine and Morphology

Ultrastructural Changes of the Aortic Walls in Conditions of Experimental Streptozotocin Induced Diabetes

Tsitovskyi M. N., Kryvko Iu. Ia., Mateshuk-Vatseba L. R., Dmytriv H. M.
Abstract

The purpose of our study was to determine the ultrastructural peculiarities of the haemomicrocirculatory pathways of the aortic walls of the albino rat in the norm and in the course of experimental diabetes mellitus (DM). Materials and methods. Using the method of electron microscopy, it was established that the aortic wall is composed of three tissue layers: the inner layer represented by the endothelial cells, the sub-endothelial layer, and the internal elastic membrane; medial layer which consists of the external elastic membrane/lamina; and the external (outer) layer -adventitia. The latter is formed by the loose fibrous connective tissue with a small amount of collagen fibers, muscle cells and macrophages. Haemomicrocirculatory pathway vessels (HPV) start from the blood vessels located in the adventitia, penetrate the outer third of the medial layer and branch out between the outer and medial layers of the aortic wall. Results and discussion. The first ultrastructural changes in the organization of the aortic wall and its parts of the HPV are observed after two weeks of the onset of streptozotocin induced diabetes and are gradually increasing with time. Destructive changes in diabetes are manifested by the restructuring of the ultrastructure of all layers of the aortic wall. The question "to be or not to be" for atherosclerosis is predetermined by the interaction of dislipoproteinemia of an atherogenic nature with the vascular wall. Endothelium plays an important role in these processes. Complex functional-adaptive features of the endothelium provide support of vascular homeostasis at a certain level and prevent damage to cells and fibers. Loss of these mechanisms leads to a violation of the vessel's permeability, namely, by the components of plasma, and the development of atherosclerosis or arteriosclerosis, depending on the factor causing this damage. One of the key issues in the pathogenesis of atherosclerotic lesions of the vascular wall in diabetes is the analysis of the mechanisms and routes of transport of atherogenic lipoproteins in the vascular wall through the endothelium, which acts as the only cell barrier between blood plasma and intima of the vessel. Studying the ultrastructure of the elastic component of the aorta medial layer, there was observed a loss of normal elastic architecture of the aortic mediums. Conclusions. Disorganized and disordered elastic fibers were found that linked the lumbar units, which caused a local weakening of the aortic wall. This work is the basis for further research of morphologists and clinicians regarding the development of new diagnostic, preventive and treatment methods for diabetic angiopathy in patients with diabetes. The study is a part of the research work of the Lviv National Medical University named after Danylo Halytsky on "Morphological peculiarities of the haemomicrocirculatory pathway of the aortic wall of the rat in norm and in experimental diabetes mellitus".

Keywords: aorta, ultrastructure, diabetes mellitus, Wistar rat

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References
  1. Tsytovskyi MN. Statystychnyi, klinichnyi ta morfolohichnyi aspekty vplyvu tsukrovoho diabetu na stan sertsevo-sudynnoi systemy. Naukovyi visnyk Uzhhorodskoho universytetu. Seriya Medytsyna. 2017; 1 (55): 168-77. [Ukrainian]
  2. Saltykov BB, Velikov VK. Dinamicheskoe morfologicheskoe nablyudenie za razvitiem diabeticheskoy mikroangiopatii. Arkhiv patologii. 2000; 6: 42-6. [Russian]
  3. Kikhtyak OP, Skrypnyk NV. Mozhlyvosti vidtvorennya tsukrovoho diabetu v eksperymenti. Eksperymentalna ta klinichna fiziolohiya ta biokhimiya. 2004; 2: 118-20. [Ukrainian]
  4. Yarek-Martynova IR, Shestakova MV. Sakharnyy diabet i endotelialnaya disfunktsiya. Sakharnyy diabet. 2004; 2: 48-52. [Russian]
  5. Novikova EG, Titova GP, Galankina IE. Morfologicheskie izmeneniya stenki aorty pri rasslaivayushchey anevrizme. Arkhiv patologii. 2013; 75 (6): 3-8. [Russian]
  6. Yakovtsova II, Topchiy II, Danilyuk SV, Kirienko AN, Kirienko DA. Morfologicheskie osobennosti stroeniya aorty, koronarnykh arteriy i miokarda pri khronicheskoy bolezni pochek. ScienceRise. Medical science. 2015; 12.3 (17): 66-71. [Russian]
  7. Soguyko YuR, Krivko YuYa, Krikun EN, Novikov OO. Morfofunktsionalnaya kharakteristika pecheni krys v norme i pri sakharnom diabete v eksperimente. Sovremennye problemy nauki i obrazovaniya: elektron nauch zhurn. 2013; 1: 1-7. Available from: http://dspace.bsu.edu.ru/handle/123456789/7642. [Russian]
  8. Mateshuk-Vatseba LR, Sultan RYa. Osoblyvosti ultrastrukturnoi orhanizatsiyi epiteliyu slyzovoi obolonky yazyka za umov tsukrovoho diabetu v eksperymenti. Naukovyi visnyk Uzhhorodskoho universytetu. Seriya Medytsyna. 2016; 1: 24-8. [Ukrainian]
  9. Нagan PG, Nienaber CA, Isselbacher EM, Bruckman D, Karavite DJ, Russman PL, et al. The international registry of acute aortic dissectional (IRAD): new insights into an old disease. JAMA. 2000 Feb 16; 283 (7) :897-903. https://www.ncbi.nlm.nih.gov/pubmed/10685714
  10. Kemm AD, Lyusher TF, Serrius PV, Eds. Bolezni serdtsa i sosudov. Rukovodstvo Evropeyskogo obshchestva kardiologov: per s angl Shlyakhto EV. Moskva: GEOTAR-Media; 2011. 1480 с. [Russian]
  11. Herrera RN, Miotti JA, Pereyra AS, Lobo MV, Ibarra MT, Tomé Guzmán AF. Marfan syndrome association with aortic dissection, venus thromboembolism and hyperhomocesteinemia. Medicina (B Aires). 2012; 72 (6): 478-80.
  12. Cury M, Zeidan F, Lobato AC. Aortic disease in the young: genetic aneurysm syndromes, connective tissue disorders, and familial aortic aneurysms and dissections. Int J Vasc Med. 2013 Jan 14; 2013: 267215. https://www.ncbi.nlm.nih.gov/pubmed/23401778. https://www.ncbi.nlm.nih.gov/pmc/articles/3557640. https://doi.org/10.1155/2013/267215
  13. Wang L, Zhang J, Fu W, Guo D, Jiang J, Wang Y. Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection. J Vasc Surg. 2012 Dec; 56 (6): 1698-709. https://www.ncbi.nlm.nih.gov/pubmed/22960022. https://doi.org/10.1016/j.jvs.2012.05.084