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УЖМБС 2017, 2(5): 116–119
https://doi.org/10.26693/jmbs02.05.116
Hygiene and Ecology

Comparative Research of Osteoassociated Micronutrient Levels of Copper in Bone Tissue under the Influence of Lead in Macro and Nanoquenchhelate Form on Animals in Experimental Conditions

Biletskaya E., Kalinicheva V.
Abstract

Nowadays population’s morbidity connected with bone and muscular system diseases increases rapidly. In addition, there is a tendency of skeleton’s strength reducing and technogenic pressure on the human body increasing. All these factors determine the need for hygienic analysis of the osteoassociated trace elements role, including copper, in the development of osteopathy in the industrial city population. Bone tissue has the highest cumulative properties in the human body, in relation to many xenobiotics, in particular to the group of heavy metals, among which lead is leading in the degree of bone tissue affinity. Essential micronutrients, including copper, play an important role in bone remodeling. Thus, copper is an activator of bone mineralization and is involved in the synthesis of organic substances for bone formation. In this regard, the purpose of the study is to investigate the effect of the isolated action of lead compounds in the macro- and nanoacquale form on the content of copper in the bone tissue of laboratory animals under experimental conditions. Materials and methods. Wistar rats were selected for experimental research. The experiment was conducted by means of methodological approaches that meet the current international requirements for toxicological experiments using animals in accordance with the European Convention. Animals were randomly assigned to two subjects and one control group with homogeneity in average weight. The dose of lead acetate (macrophage of lead) is close to 1/30000 LD50, corresponds to 0.05 mg/kg body weight, administered in isolation (the second experimental group) and lead citrate (nanoacqualate form of lead) - at a dose of 0.05 mg/kg (the third experimental group). Rats of the control group (the first group) got distilled water at the same time. For the maximum approximation of the experiment to the natural conditions, the oral route of administration according to the methodological recommendations for the study of the toxicity of metals - by the intragastric probe was chosen. The strand bone was prepared and isolated according to generally accepted techniques. Research results and its discussion. It was found out that the copper content in the bone tissue was lower than in the control group of animals, under the conditions of low-dose exposure to lead. Thus, the copper concentration decreased by 43.5% (p<0.01) and was 3.87 ± 0.28 mg/kg compared with the control group, the similar figures of which correspond to 6.85 ± 0.7 mg/kg. The resulting effect contributes to a violation of the ratio Zn: Cu, due to copper deficiency, which, in turn, affects the concentration of zinc, proven lead bioanagonist. Consequently, the cascade of violations of the bones’ mineral composition can cause certain morphological changes and development of osteopathies. Among the animals of the third group, the content of the investigated microelement decreased by 44.8% (p <0.01) and was 3.78 ± 0.15 mg/kg compared to the bone tissue of the control group rats. The comparative research of experimental groups indicated that there was no significant difference between the effect of the macro- and nanoacqualate form of lead on the copper content in the bone tissue, that is, lead acetate and lead citrate almost equally contribute to a decrease in the bone content of copper in the average by 44.2%. Results. During isolated low-dose exposure of lead acetate (lead macrophage) and lead citrate (nanoacquale form) copper content in bone tissue decreases to 43.5% (p<0.01) and 44.8% (p<0.01) respectively. The results of the copper reduction, breaking the ratio Zn: Cu, due to a significant deficit of the latter, affects the concentration of zinc, which is proven lead bioanagonist.

Keywords: lead, micronutrients, bone tissue

Full text: PDF (Ukr) 207K

References
  1. Biletska EM, Onul NM, Kalinicheva VV. Porivnyalna otsinka bioprotektornoi diyi tsynku v makro- ta nanoakvakhelatniy formi na osteotropnist svyntsyu v eksperymentalnykh umovakh. Zhurnal «Medychni perspektyvy». 2016; 21 (4): 123-9. [Ukrainian].
  2. Elizarova ON, Zhidkova LV, Kochetkova TA. Posobie po toksikologii dlya laborantov. Moskva: Meditsina, 1974. 168 s. [Russian].
  3. Lemesheva SA. Khimicheskiy sostav, svoystva kostnogo apatita i ego analogov: avtoref. dis. … kand. chem. Nauk. Abstr. PhDr. (Chem.). Moskva; 2010. 20 s. [Russian].
  4. Antonovich EA, Kagan YuS, Spynu EI. Metodicheskie ukazaniya po gigienicheskoy otsenke novykh pestitsidov. Ministerstvo zdravookhraneniya SSSR; VNII gigieny i toksikologii pestitsidov, polimerov i plasticheskikh mass. Kiev, 1988. [Russian].
  5. Chen Zh, Salam MT, Karim R, Toledo-Corral CM, Watanabe RM, Xiang AH, Buchanan TA, Habre R, Bastain TM, Lurmann F, Taher M, Wilson JP, Trigo E, Gilliland FD. Living near a Freeway is Associated with Lower Bone Mineral Density among Mexican Americans. Osteoporos Int. 2015 Jun; 26 (6): 1713–21. https://www.ncbi.nlm.nih.gov/pubmed/25677718. https://www.ncbi.nlm.nih.gov/pmc/articles/4470808 https://doi.org/10.1007/s00198-015-3051-z
  6. Allen LH. Food Safety: Heavy Metals. Encyclopedia of Human Nutrition. Third Edition. 2013. p. 331-6.
  7. Sierpinska T, Konstantynowicz J, Orywal K, Golebiewska M, Szmitkowski M. Copper deficit as a potential pathogenic factor of reduced bone mineral density and severe tooth wear. Osteoporosis International. 2014; 25 (2): 447-54. https://www.ncbi.nlm.nih.gov/pubmed/23797848. https://www.ncbi.nlm.nih.gov/pmc/articles/3906556. https://doi.org/10.1007/s00198-013-2410-x
  8. Lu H, Yuan G, Yin Z, Dai S, Jia R, Xu J, Song X, Li L, Lv C. Effects of subchronic exposure to lead acetate and cadmium chloride on rat’s bone: Ca and Pi contents, bone density, and histopathological evaluation. Int J Clin Exp Pathol. 2014; 7: 640-7. https://www.ncbi.nlm.nih.gov/pubmed/ 24551284. https://www.ncbi.nlm.nih.gov/pmc/articles/3925908
  9. Smith JT, Schneider AD, Katchko KM, Yun C, Hsu EL. Environmental Factors Impacting Bone-Relevant Chemokines. Front Endocrinol (Lausanne). 2017; 8: 22. https://www.ncbi.nlm.nih.gov/pubmed/28261155. https://www.ncbi.nlm.nih.gov/pmc/articles/5306137. https://doi.org/10.3389/fendo.2017.00022
  10. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasburg, 1986. 53 p.
  11. Chongwatpol P, Rendina-Ruedy E, Stoecker BJ. Implications of compromised zinc status on bone loss associated with chronic inflammation in C57BL/6 mice. Journal Inflammation Research. 2015; 8: 117–28. https://www.ncbi.nlm.nih.gov/pmc/articles/4508086. https://doi.org/10.2147/JIR.S82261