ISSN 2415-3060 (print), ISSN 2522-4972 (online)
  • 23 of 41
Up
УЖМБС 2017, 2(3): 135–139
https://doi.org/10.26693/jmbs02.03.135
Clinical Medicine

Comparative Analysis of the Tubes for Obtaining Platelet Enriched Plasma

Sulaieva O.N.
Abstract

Platelet enriched plasma is considered as one of the best ways to stimulate tissue repair and age associated changes correction. The widespread use of PRP therapy in various fields of medicine requires compliance with the requirements for medical devices which are used to obtain PRP. The purpose of the article is to examine the compliance of different types of PRP-tubes in terms of safety and efficacy criteria. Six types of tubes that are available in Ukrainian market were analyzed: Endoret (BTI), Plasmolifting, RegenLab, MM Medic, PlasmoMed and Ycellbio. According to the construction and protocol of PRP obtaining these tubes were subdivided into 3 groups: 1) simple tubes (BTI and MM Medic) that include only anticoagulant, 2) tubes with separating gel (Plasmolifting and RegenLab) and 3) tubes with a special construction facilitating PRP isolation. The following characteristics were taken into account during the study: the material from which the tubes were made, tube’s components, type of anticoagulant, centrifugation protocol, and platelets count in plasma. The highest compliance with the international recommendations for PRP-therapy was shown for Endoret (BTI), MM Medic and Ycellbio tubes. PRP with an optimal count of platelets (1 million per mkl) can be obtained with simple tubes (MM Medic, Endoret BTI). Using tubes with a special configuration (Ycellbio) facilitated the selection of plasma, but number of platelets in PRP was excessive. One more disadvantage of this type of tubes is the systems openness and the risk of contamination of the biomaterial. The plasma obtained with gel-containing tubes (RegenLab, Plasmolifting) does not differ significantly from the whole blood in terms of platelets count blood. In addition, the presence of gel requires the increase of centrifugation acceleration that could lead to spontaneous activation of platelets. Standardization of PRP obtaining characteristics will be useful for further development of regenerative medicine technologies.

Keywords: PRP-therapy, platelets, plasma, test tubes

Full text: PDF (Rus) 212K

References
  1. Agrawal А. А. Evolution, current status and advances in application of platelet concentrate in periodontics and implantology. World J Clin Cases. 2017; 5 (5): 159–71. https://www.ncbi.nlm.nih.gov/pubmed/28560233. https://www.ncbi.nlm.nih.gov/pmc/articles/5434315. https://doi.org/10.12998/wjcc.v5.i5.159
  2. Dhurat R, Sukesh MS. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author's Perspective. J Cutan Aesthet Surg. 2014; 7 (4): 189–97. https://www.ncbi.nlm.nih.gov/pubmed/25722595. https://www.ncbi.nlm.nih.gov/pmc/articles/4338460. https://doi.org/10.4103/0974-2077.150734
  3. Kushida S, Kakudo N, Morimoto N, Hara T, Ogawa T, Mitsui T, Kusumoto K. Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial separation systems. J Artif Organs. 2014; 17 (2): 186-92. https://www.ncbi.nlm.nih.gov/pubmed/24748436. https://doi.org/10.1007/s10047-014-0761-5
  4. Martinez-Zapata MJ, Martí-Carvajal AJ, Solà I, Expósito JA, Bolíbar I, Rodríguez L, Garcia J, Zaror C. Autologous platelet-rich plasma for treating chronic wounds. Cochrane Database Syst Rev. 2016; 25 (5): CD006899. https://www.ncbi.nlm.nih.gov/pubmed/27223580. https://doi.org/10.1002/14651858.CD006899.pub3
  5. Perez AGM, Lana SD, Rodrigues AA, Luzo ACM, Belangero WD, Santana MHA. Relevant Aspects of Centrifugation Step in the Preparation of Platelet-Rich Plasma. ISRN Hematol. 2014; 2014: 176060. http://dx.doi.org/10.1155/2014/176060
  6. Platelet Rich Plasma (PRP) Guidelines, © International Cellular Medicine Society - 2011. Available from: http://www.cellmedicinesociety.org
  7. Sister D. PRP: the new frontier in regenerative medicine and aesthetic medicine. Firence; 2016. 158 p.
  8. Ehrenfest DM, Andia I, Zumstein MA, Zhang CQ, Pinto NR, Bielecki T. Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives. Muscles Ligaments Tendons J. 2014; 4 (1): 3–9. https://www.ncbi.nlm.nih.gov/pubmed/24932440. https://www.ncbi.nlm.nih.gov/pmc/articles/4049647
  9. Jubert JN, Rodríguez L, Reverté-Vinaixa MM, Navarro A. Platelet-Rich Plasma Injections for Advanced Knee Osteoarthritis: A Prospective, Randomized, Double-Blinded Clinical Trial. Orthop J Sports Med. 2017; 5 (2): 2325967116689386. https://www.ncbi.nlm.nih.gov/pubmed/28255569. https://www.ncbi.nlm.nih.gov/pmc/articles/5315239. https://doi.org/10.1177/2325967116689386